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Counting White Blood Cells from a Blood Smear Using Fourier Ptychographic Microscopy

机译：使用傅里叶液相色谱显微镜对血涂片中的白细胞进行计数

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摘要

White blood cell (WBC) count is a valuable metric for assisting with diagnosis or prognosis of various diseases such as coronary heart disease, type 2 diabetes, or infection. Counting WBCs can be done either manually or automatically. Automatic methods are capable of counting a large number of cells to give a statistically more accurate reading of the WBC count of a sample, but the specialized equipment tends to be expensive. Manual methods are inexpensive since they only involve a conventional light microscope setup. However, it is more laborious and error-prone because the small field-of-view (FOV) of the microscope necessitates mechanical scanning of a specimen for counting an adequate number of WBCs. Here, we investigate the use of Fourier ptychographic microscopy (FPM) to bypass these issues of the manual methods. With a 2x objective, FPM can provide a FOV of 120 mm2 with enhanced resolution comparable to that of a 20x objective, which is adequate for non-differentially counting WBCs in just one FOV. A specialist was able to count the WBCs in FPM images with 100% accuracy compared to the count as determined from conventional microscope images. An automatic counting algorithm was also developed to identify WBCs from FPM’s captured images with 95% accuracy, paving the way for a cost-effective WBC counting setup with the advantages of both the automatic and manual counting methods.
机译：白细胞（WBC）计数是有助于诊断或预测各种疾病（例如冠心病，2型糖尿病或感染）的重要指标。白细胞计数可以手动或自动进行。自动方法能够对大量的细胞进行计数，以统计地更准确地读取样品的WBC计数，但是专用设备往往很昂贵。手动方法便宜，因为它们仅涉及常规的光学显微镜设置。但是，这比较费力且容易出错，因为显微镜的小视场（FOV）要求对样品进行机械扫描以计数足够数量的WBC。在这里，我们调查使用傅立叶色谱分析（FPM）来绕过手动方法的这些问题。使用2倍物镜，FPM可以提供120 mm 2 的FOV，其分辨率与20倍物镜的分辨率相当，这足以在一个FOV中对WBC进行非差分计数。与传统显微镜图像确定的计数相比，专家能够以100％的精度对FPM图像中的白细胞进行计数。还开发了一种自动计数算法，可以以95％的精度从FPM捕获的图像中识别WBC，从而为具有成本效益的WBC计数设置铺平了道路，它具有自动计数方法和手动计数方法的优点。

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