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首页> 美国卫生研究院文献>Frontiers in Plant Science >Structure expression profile and phylogenetic inference of chalcone isomerase-like genes from the narrow-leafed lupin (Lupinus angustifolius L.) genome
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Structure expression profile and phylogenetic inference of chalcone isomerase-like genes from the narrow-leafed lupin (Lupinus angustifolius L.) genome

机译：窄叶羽扇豆（Lupinus angustifolius L.）基因组中查尔酮异构酶样基因的结构表达谱和系统发育推断

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Lupins, like other legumes, have a unique biosynthesis scheme of 5-deoxy-type flavonoids and isoflavonoids. A key enzyme in this pathway is chalcone isomerase (CHI), a member of CHI-fold protein family, encompassing subfamilies of CHI1, CHI2, CHI-like (CHIL), and fatty acid-binding (FAP) proteins. Here, two Lupinus angustifolius (narrow-leafed lupin) CHILs, LangCHIL1 and LangCHIL2, were identified and characterized using DNA fingerprinting, cytogenetic and linkage mapping, sequencing and expression profiling. Clones carrying CHIL sequences were assembled into two contigs. Full gene sequences were obtained from these contigs, and mapped in two L. angustifolius linkage groups by gene-specific markers. Bacterial artificial chromosome fluorescence in situ hybridization approach confirmed the localization of two LangCHIL genes in distinct chromosomes. The expression profiles of both LangCHIL isoforms were very similar. The highest level of transcription was in the roots of the third week of plant growth; thereafter, expression declined. The expression of both LangCHIL genes in leaves and stems was similar and low. Comparative mapping to reference legume genome sequences revealed strong syntenic links; however, LangCHIL2 contig had a much more conserved structure than LangCHIL1. LangCHIL2 is assumed to be an ancestor gene, whereas LangCHIL1 probably appeared as a result of duplication. As both copies are transcriptionally active, questions arise concerning their hypothetical functional divergence. Screening of the narrow-leafed lupin genome and transcriptome with CHI-fold protein sequences, followed by Bayesian inference of phylogeny and cross-genera synteny survey, identified representatives of all but one (CHI1) main subfamilies. They are as follows: two copies of CHI2, FAPa2 and CHIL, and single copies of FAPb and FAPa1. Duplicated genes are remnants of whole genome duplication which is assumed to have occurred after the divergence of Lupinus, Arachis, and Glycine.
机译：羽扇豆与其他豆科植物一样，具有独特的5-脱氧型黄酮和异黄酮的生物合成方案。此途径中的关键酶是查耳酮异构酶（CHI），它是CHI折叠蛋白家族的成员，涵盖CHI1，CHI2，CHI样（CHIL）和脂肪酸结合（FAP）蛋白的亚家族。在这里，使用DNA指纹图谱，细胞遗传学和连锁作图，测序和表达谱分析，鉴定并鉴定了两个羽扇豆（窄叶羽扇豆）CHILs LangCHIL1和LangCHIL2。携带CHIL序列的克隆被组装成两个重叠群。从这些重叠群获得完整的基因序列，并通过基因特异性标记将其映射在两个古铜色链霉菌连锁群中。细菌人工染色体荧光原位杂交方法证实了两个LangCHIL基因在不同染色体中的定位。两种LangCHIL亚型的表达谱非常相似。转录水平最高的是植物生长的第三周。此后，表达下降。 LangCHIL基因在叶和茎中的表达相似且低。比较映射到参考豆类基因组序列揭示了很强的同义链接。但是，LangCHIL2重叠群的结构比LangCHIL1保守得多。假定LangCHIL1是祖先基因，而LangCHIL1可能是重复的结果。由于两个拷贝都具有转录活性，因此就它们的假设功能差异提出了疑问。筛选具有CHI折叠蛋白序列的羽扇豆羽扇豆基因组和转录组，然后通过系统的贝叶斯推断和跨世代同义调查，确定了一个（CHI1）主要亚科的代表。它们如下：CHI2的两个副本 FAPa2 和 CHIL ，以及 FAPb 和 FAPa1 的单个副本。重复的基因是整个基因组重复的残基，假定发生在 Lupinus ， Arachis 和 Glycine 发散之后。

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