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Recent advances in the Zymoseptoria tritici–wheat interaction: insights from pathogenomics

机译:小麦酵母菌-小麦相互作用的最新进展:病原学研究的见解

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摘要

We examine the contribution of next generation sequencing (NGS) to our understanding of the interaction between the fungal pathogen Zymoseptoria tritici and its wheat host. Recent interspecific whole genome comparisons between Z. tritici and its close relatives provide evidence that Z. tritici has undergone strong adaptive evolution, which is attributed to specialization by Z. tritici on wheat. We also assess the contribution of recent RNA sequencing datasets toward identifying pathogen genes and mechanisms critical for disease. While these studies have yet to report a major effector gene, they illustrate that assembling reads to the reference genome is a robust method to identify fungal transcripts from in planta infections. They also highlight the strong influence that the wheat cultivar has on effector gene expression. Lastly, we suggest future directions for NGS-guided approaches to address largely unanswered questions related to cultivar and lifecycle dependent gene expression and propose that future experiments with Z. tritici be conducted on a single wheat cultivar to enable comparisons across experiments.
机译:我们研究了下一代测序(NGS)对我们了解真菌病原体Zymoseptoria tritici及其小麦宿主之间相互作用的贡献。最近小麦和其近亲之间的种间全基因组比较提供了证据,表明小麦已经发生了强适应性进化,这归因于小麦对小麦的特化。我们还评估了最近的RNA测序数据集对鉴定病原体基因和对疾病至关重要的机制的贡献。尽管这些研究尚未报告主要的效应基因,但它们表明,将读段组装到参考基因组是从植物感染中鉴定真菌转录本的可靠方法。他们还强调了小麦品种对效应子基因表达的强大影响。最后,我们提出了NGS指导的方法的未来方向,以解决与品种和生命周期相关的基因表达有关的大部分未回答的问题,并建议在单个小麦品种上进行Z. tritici的未来实验,以便进行整个实验的比较。

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