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Co-Cultivation of Fungal and Microalgal Cells as an Efficient System for Harvesting Microalgal Cells Lipid Production and Wastewater Treatment

机译:真菌和微藻细胞的共培养是收获微藻细胞脂质生产和废水处理的有效系统

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摘要

The challenges which the large scale microalgal industry is facing are associated with the high cost of key operations such as harvesting, nutrient supply and oil extraction. The high-energy input for harvesting makes current commercial microalgal biodiesel production economically unfeasible and can account for up to 50% of the total cost of biofuel production. Co-cultivation of fungal and microalgal cells is getting increasing attention because of high efficiency of bio-flocculation of microalgal cells with no requirement for added chemicals and low energy inputs. Moreover, some fungal and microalgal strains are well known for their exceptional ability to purify wastewater, generating biomass that represents a renewable and sustainable feedstock for biofuel production. We have screened the flocculation efficiency of the filamentous fungus A. fumigatus against 11 microalgae representing freshwater, marine, small (5 µm), large (over 300 µm), heterotrophic, photoautotrophic, motile and non-motile strains. Some of the strains are commercially used for biofuel production. Lipid production and composition were analysed in fungal-algal pellets grown on media containing alternative carbon, nitrogen and phosphorus sources contained in wheat straw and swine wastewater, respectively. Co-cultivation of algae and A. fumigatus cells showed additive and synergistic effects on biomass production, lipid yield and wastewater bioremediation efficiency. Analysis of fungal-algal pellet's fatty acids composition suggested that it can be tailored and optimised through co-cultivating different algae and fungi without the need for genetic modification.
机译:大型微藻产业面临的挑战与关键作业(如收获,营养供应和石油提取)的高昂成本有关。用于捕捞的高能量输入使得当前的商业化微藻生物柴油生产在经济上不可行,并可占生物燃料生产总成本的50%。真菌和微藻细胞的共培养越来越受到关注,因为微藻细胞的生物絮凝效率很高,不需要添加化学药品和低能量输入。此外,一些真菌和微藻菌株以其净化废水,产生代表生物燃料生产的可再生和可持续原料的生物质的卓越能力而闻名。我们已经筛选了丝状真菌烟曲霉对11种微藻的淡水絮凝效率,这些微藻代表淡水,海洋,小型(5 µm),大型(超过300 µm),异养,光合自养,能动和非能动菌株。一些菌株在商业上用于生物燃料生产。分析了在分别含有麦秸和猪废水中碳,氮和磷替代源的培养基上生长的真菌-藻类沉淀物中的脂质产生和组成。藻类和烟曲霉细胞的共培养显示出对生物量产生,脂质产量和废水生物修复效率的累加和协同作用。对真菌-藻类沉淀物的脂肪酸组成的分析表明,可以通过共培养不同的藻类和真菌来进行定制和优化,而无需进行基因改造。

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