• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Experimental Medicine >Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyers patches that switch sIgM B Cells to sIgA B cells in vitro
【2h】

Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyers patches that switch sIgM B Cells to sIgA B cells in vitro

机译：调节小鼠肠道相关淋巴组织中IgA类特异性免疫球蛋白产生的机制。 I.从Peyer贴片衍生的T细胞可在体外将sIgM B细胞转换为sIgA B细胞

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To explore mechanisms of T cell regulation governing mucosal IgA immune response, concanavalin A-induced cloned T cell lines from Peyer's patches (PP) as well as spleen were established. The cloned cell lines expressed Thy- 1.2(+), Lyt-l(+)2(-) and were radioresistant (1,500 rad). The capacity of the cloned T cells to regulate Ig synthesis was determined by measuring their effect on lipopolysaccharide (LPS)-induced polyclonal Ig synthesis by PP B cells. In initial studies Ig secreted by B cells was determined by double antibody radioimmunoassay. LPS in the absence of cloned T cells induced abundant amounts of IgM (average 8,860 ng/2 × 10(5) B cells) and IgG (average 1,190 ng/2 × 10(5) B cells), but little or no IgA. The addition of PP cloned T cells markedly suppressed production of IgM (88 percent at the highest T/B cell ratio, 4:1), but the addition of spleen cloned T cells suppressed only a little or not at all. IgG production was inhibited by both PP and spleen T clone cells (70 percent at the 4:1 T/B ratio), wheras IgA synthesis was enhanced by both clones, but only to a limited degree. In subsequent studies the expression of class-specific surface Ig (sIg) and cytoplasmic Ig (cIg) on/in unseparated PP B cells as well as Ig class- specific PP B cells and spleen B cells during culture with or without the cloned T cells was determined by immunofluorescence. The major findings were as follows: (a) Compared with unseparated B cell cultures and cultures of purified sIgM B cells derived from PP containing LPS alone, cultures containing LPS and PP cloned T cells showed a marked decrease in cIgM-, sIgG-, and cIgG-expressing cells that was accompanied by a striking increase in sIgA-bearing, but not cIgA-containing, cells. In contrast, unseparated B cell cultures and cultures of purified sIgM B cells derived from PP containing LPS and spleen cloned T cells did not show any increase in sIgA- bearing cells. (b) Compared with purified sIgG-bearing PP B cell cultures containing LPS alone, purified sIgG-bearing PP B cell cultures containing both LPS and PP cloned T cells showed no substantial change in sIgG- or cIgG- expressing cells, and no sIgA- or cIgA- expressing cells appeared. (c) Compared with sIgA-bearing PP B cell cultures containing LPS alone, purified sIgA-bearing PP B cell cultures containing both LPS and PP cloned T cells showed no increased proliferation, and cIgA cells did not occur. Cultures of purified sIgM B cells derived from spleen containing LPS and PP cloned T cells showed qualitatively similar changes. From these results we conclude that PP cloned T cells induced class-specific switching from sIgM- to sIgA- bearing B cells, whereas spleen cloned T cells lacked this property, although they may have induced an IgM {arrow} IgG or intersubclass IgG switch. These processes seem to be in part tissue dependent. Furthermore, the PP switch T cells appear to operate as true switch cells, which govern the pathway of DNA recombination events, rather than as classical helper cells, which act to expand already differentiated cells. Finally, these switch T cells probably account for the fact that PP are an important source of IgA B cells and also a major site of IgA heavy chain class switching during gut-associated mucosal B cell proliferation and differentation.
机译：为了探索控制粘膜IgA免疫反应的T细胞调节机制，建立了伴刀豆球蛋白A诱导的从Peyer's贴片（PP）和脾脏克隆的T细胞系。克隆的细胞系表达Thy-1.2（+），Lyt-1（+）2（-），并且具有放射抗性（1,500 rad）。克隆的T细胞调节Ig合成的能力是通过测量它们对PP B细胞对脂多糖（LPS）诱导的多克隆Ig合成的影响来确定的。在最初的研究中，通过双抗体放射免疫测定法确定了B细胞分泌的Ig。没有克隆T细胞的LPS诱导大量IgM（平均8,860 ng / 2×10（5）B细胞）和IgG（平均1,190 ng / 2×10（5）B细胞），但几乎没有IgA。 PP克隆的T细胞的添加显着抑制了IgM的产生（在最高T / B细胞比例为4：1时为88％），但脾脏克隆的T细胞的添加仅抑制了一点甚至完全没有。 PP和脾T克隆细胞均抑制了IgG的产生（T / B比例为4：1时为70％），两个克隆均增强了小麦IgA的合成，但程度有限。在随后的研究中，在有或没有克隆T细胞的培养过程中，未分离的PP B细胞上/中的类特异性表面Ig（sIg）和细胞质Ig（cIg）以及Ig类特异性PP B细胞和脾B细胞的表达通过免疫荧光测定。主要发现如下：（a）与未分离的B细胞培养物和仅包含LPS的PP衍生的纯化sIgM B细胞培养物相比，包含LPS和PP克隆的T细胞的培养物显示cIgM-，sIgG-和cIgG表达细胞，伴随sIgA的细胞显着增加，但不包含cIgA的细胞显着增加。相反，未分离的B细胞培养物和纯化的sIgM B细胞的培养物（来自含LPS的PP和脾脏克隆的T细胞）未显示出携带sIgA的细胞的增加。 （b）与仅包含LPS的纯化的带有sIgG的PP B细胞培养物相比，同时包含LPS和PP克隆的T细胞的纯化的带有sIgG的PP B细胞培养物在表达sIgG或cIgG的细胞中没有实质性变化，并且没有sIgA-或表达cIgA的细胞出现了。 （c）与仅包含LPS的带有sIgA的PP B细胞培养物相比，同时包含LPS和PP克隆的T细胞的纯化的带有sIgA的PP B细胞培养物显示没有增加的增殖，并且没有出现cIgA细胞。从含有LPS和PP克隆的T细胞的脾脏中纯化的sIgM B细胞的培养在质上表现出相似的变化。根据这些结果，我们得出结论，PP克隆的T细胞诱导了从sIgM-到sIgA的B细胞的类特异性转换，而脾克隆的T细胞缺乏这种特性，尽管它们可能已经诱导了IgM {arrow} IgG或亚类IgG转换。这些过程似乎部分取决于组织。此外，PP开关T细胞似乎起着控制DNA重组事件途径的真正的开关细胞的作用，而不是作为传统的辅助细胞发挥作用，传统的辅助细胞起着已经分化的细胞的作用。最后，这些开关T细胞可能解释了PP是肠道相关粘膜B细胞增殖和分化过程中IgA B细胞的重要来源，也是IgA重链类转换的主要部位。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号