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Physical Mapping of the 5S and 18S rDNA in Ten Species of Hypostomus Lacépède 1803 (Siluriformes: Loricariidae): Evolutionary Tendencies in the Genus

机译:十种HypostomusLacépède1803(Siluriformes:Loricariidae)中5S和18S rDNA的物理图谱:属的进化趋势

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摘要

Hypostomus is a diverse group with unclear aspects regarding its biology, including the mechanisms that led to chromosome diversification within the group. Fluorescence in situ hybridization (FISH) with 5S and 18S rDNA probes was performed on ten Hypostomini species. Hypostomus faveolus, H. cochliodon, H. albopunctatus, H. aff. paulinus, and H. topavae had only one chromosome pair with 18S rDNA sites, while H. ancistroides, H. commersoni, H. hermanni, H. regani, and H. strigaticeps had multiple 18S rDNA sites. Regarding the 5S rDNA genes, H. ancistroides, H. regani, H. albopunctatus, H. aff. paulinus, and H. topavae had 5S rDNA sites on only one chromosome pair and H. faveolus, H. cochliodon, H. commersoni, H. hermanni, and H. strigaticeps had multiple 5S rDNA sites. Most species had 18S rDNA sites in the telomeric region of the chromosomes. All species but H. cochliodon had 5S rDNA in the centromeric/pericentromeric region of one metacentric pair. Obtained results are discussed based on existent phylogenies for the genus, with comments on possible dispersion mechanisms to justify the variability of the rDNA sites in Hypostomus.
机译:Hypostomus是一个多样化的群体,其生物学方面尚不清楚,包括导致该群体内染色体多样化的机制。用5S和18S rDNA探针进行荧光原位杂交(FISH),对10个Hypostomini物种进行了检测。 Hypostomus faveolus,H。cochliodon,H。albopunctatus,H。aff。 paulinus和H. topavae只有一个染色体对具有18S rDNA位点,而ancistroides,H。commersoni,H.hermanni,H。regani和H. strigaticeps具有多个18S rDNA位点。关于5S rDNA基因,H。ancistroides,H。regani,H。albopunctatus, H。等。 paulinus H。 topavae 仅在一个染色体对和 H上具有5S rDNA位点。 faveolus H。耳蜗 H。 commersoni H。 hermanni H。股癣有多个5S rDNA位点。大多数物种在染色体的端粒区域具有18S rDNA位点。除 H外的所有物种。耳蜗动物在一个亚中心对的着丝粒/周围异构体区域具有5S rDNA。讨论了基于该属的系统发育史所获得的结果,并评论了可能的分散机制以证明 Hypostomus 中rDNA位点的变异性。

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