• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Experimental Medicine >Macrophage type 3 complement receptors mediate serum-independent binding of Leishmania donovani. Detection of macrophage-derived complement on the parasite surface by immunoelectron microscopy
【2h】

Macrophage type 3 complement receptors mediate serum-independent binding of Leishmania donovani. Detection of macrophage-derived complement on the parasite surface by immunoelectron microscopy

机译：巨噬细胞3型补体受体介导多形利什曼原虫的血清非依赖性结合。免疫电子显微镜检测寄生虫表面巨噬细胞来源的补体

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, direct visual evidence for local opsonization of L. donovani by macrophage (M phi)-derived complement components was obtained using immunoelectron microscopy. C3 deposition was detected on the surface of both promastigotes and amastigotes after 20 min serum- free incubation with murine resident peritoneal M phi (RPM), followed by fixation and incubation first with specific antibody directed against C3 and then with gold-labelled protein A. Gold deposition was not observed around either form of the parasite if the anti-C3 antibody was omitted. For promastigotes, the degree of C3 deposition under serum- free conditions was comparable with that observed in the presence of an exogenous (serum) source of C3, but did not result in the same severe damage to the parasite as did the latter. Addition of sodium salicyl hydroxamate, which prevents covalent binding of C3 to activator surfaces, abrogated promastigote binding. Hence, although the anti-C3 antibody did not distinguish between native C3 and its breakdown product iC3b, these data support our earlier conclusion that promastigote binding to the CR3 of murine RPM is complement dependent. For amastigotes, gold deposition and binding to murine RPM were not eliminated by sodium salicyl hydroxamate. The presence of normal mouse serum resulted in increased gold deposition, but did not mediate either enhanced binding to M phi or damage to the amastigote. These data suggest that a proportion of C3 binding to the amastigote surface may be via noncovalent linkages, and that the C3 bound may not be in the correct form to mediate binding to CR3.
机译：在这项研究中，使用免疫电子显微镜获得了由巨噬细胞（M phi）衍生的补体成分对L. donovani局部调理作用的直接视觉证据。在与鼠类常驻腹膜M phi（RPM）进行20分钟无血清温育后，先在前鞭毛体和无鞭毛体的表面检测到C3沉积，然后先与针对C3的特异性抗体固定，然后与金标记的蛋白A孵育。如果省略抗C3抗体，则在两种形式的寄生虫周围均未观察到金沉积。对于前鞭毛体而言，在无血清条件下的C3沉积程度与存在外源（血清）的C3来源时观察到的程度相当，但并未对寄生虫造成相同的严重破坏。水杨基异羟肟酸钠的添加防止了C3与活化剂表面的共价结合，从而消除了前鞭毛体的结合。因此，尽管抗C3抗体无法区分天然C3及其分解产物iC3b，但这些数据支持了我们先前的结论，即前鞭毛体与鼠RPM CR3的结合是补体依赖性的。对于变形虫而言，水杨基异羟肟酸钠不能消除金的沉积和与鼠RPM的结合。正常小鼠血清的存在导致金的沉积增加，但不介导与M phi的结合增强或对鞭毛体的损害。这些数据表明，一定比例的C3结合到鞭毛体表面可能是通过非共价键连接的，并且所结合的C3可能不是以正确的形式来介导与CR3的结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号