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首页> 美国卫生研究院文献>other >Abundances of Tetracycline Sulphonamide and Beta-Lactam Antibiotic Resistance Genes in Conventional Wastewater Treatment Plants (WWTPs) with Different Waste Load
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Abundances of Tetracycline Sulphonamide and Beta-Lactam Antibiotic Resistance Genes in Conventional Wastewater Treatment Plants (WWTPs) with Different Waste Load

机译：不同废物负荷的常规废水处理厂（WWTP）中四环素磺酰胺和β-内酰胺抗生素抗性基因的丰度

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Antibiotics and antibiotic resistant bacteria enter wastewater treatment plants (WWTPs), an environment where resistance genes can potentially spread and exchange between microbes. Several antibiotic resistance genes (ARGs) were quantified using qPCR in three WWTPs of decreasing capacity located in Helsinki, Tallinn, and Tartu, respectively: sulphonamide resistance genes (sul1 and sul2), tetracycline resistance genes (tetM and tetC), and resistance genes for extended spectrum beta-lactams (blaoxa-58, blashv-34, and blactx-m-32). To avoid inconsistencies among qPCR assays we normalised the ARG abundances with 16S rRNA gene abundances while assessing if the respective genes increased or decreased during treatment. ARGs were detected in most samples; sul1, sul2, and tetM were detected in all samples. Statistically significant differences (adjusted p<0.01) between the inflow and effluent were detected in only four cases. Effluent values for blaoxa-58 and tetC decreased in the two larger plants while tetM decreased in the medium-sized plant. Only blashv-34 increased in the effluent from the medium-sized plant. In all other cases the purification process caused no significant change in the relative abundance of resistance genes, while the raw abundances fell by several orders of magnitude. Standard water quality variables (biological oxygen demand, total phosphorus and nitrogen, etc.) were weakly related or unrelated to the relative abundance of resistance genes. Based on our results we conclude that there is neither considerable enrichment nor purification of antibiotic resistance genes in studied conventional WWTPs.
机译：抗生素和抗药性细菌进入废水处理厂（WWTP），在该环境中，抗药性基因可能会在微生物之间传播和交换。使用qPCR对位于赫尔辛基，塔林和塔尔图的三个能力下降的污水处理厂中的qPCR定量了几种抗生素抗性基因（磺胺抗性基因（sul1和sul2），四环素抗性基因（tetM和tetC）和抗性基因扩展光谱的β-内酰胺（blaoxa-58，blashv-34和blactx-m-32）。为避免qPCR测定之间的不一致，我们在评估治疗期间各个基因是否增加或减少的同时，将ARG丰度用16S rRNA基因丰度进行了标准化。在大多数样品中都检测到了ARG。在所有样品中均检测到sul1，sul2和tetM。仅在四例中检测到流入与流出之间的统计学差异（校正后的p <0.01）。两种较大的植物中blaoxa-58和tetC的出水值降低，而中型植物中的tetM降低。中型工厂的废水中仅有blashv-34增加。在所有其他情况下，纯化过程不会导致抗性基因的相对丰度发生显着变化，而原始丰度却下降了几个数量级。标准水质变量（生物需氧量，总磷和氮等）与抗性基因的相对丰度相关性较弱或无关。根据我们的结果，我们得出结论，在研究的常规污水处理厂中既没有大量的抗生素抗性基因富集也没有纯化。

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