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首页> 美国卫生研究院文献>other >Scx-Transduced Tendon-Derived Stem Cells (TDSCs) Promoted Better Tendon Repair Compared to Mock-Transduced Cells in a Rat Patellar Tendon Window Injury Model
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Scx-Transduced Tendon-Derived Stem Cells (TDSCs) Promoted Better Tendon Repair Compared to Mock-Transduced Cells in a Rat Patellar Tendon Window Injury Model

机译：与模拟转导的细胞相比在大鼠Pat骨腱网损伤模型中Scx转导的源自肌腱的干细胞（TDSC）促进了更好的肌腱修复

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We hypothesized that the transplantation of Scx-transduced tendon-derived stem cells (TDSCs) promoted better tendon repair compared to the transplantation of mock-transduced cells. This study thus aimed to investigate the effect of Scx transduction on the expression of lineage markers in TDSCs and the effect of the resulting cell line in the promotion of tendon repair. Rat non-GFP or GFP-TDSCs were transduced with Scx or empty lentiviral vector (Mock) and selected by blasticidin. The mRNA expressions of Scx and different lineage markers were examined by qRT-PCR. The effect of the transplantation of GFP-TDSC-Scx on tendon repair was then tested in a rat unilateral patellar tendon window injury model. The transplantation of GFP-TDSC-Mock and scaffold-only served as controls. At week 2, 4 and 8 post-transplantation, the repaired patellar tendon was harvested for ex vivo fluorescent imaging, vivaCT imaging, histology, immunohistochemistry and biomechanical test. GFP-TDSC-Scx consistently showed higher expressions of most of tendon- and cartilage- related markers compared to the GFP-TDSC-Mock. However, the effect of Scx transduction on the expressions of bone-related markers was inconclusive. The transplanted GFP-TDSCs could be detected in the window wound at week 2 but not at week 4. Ectopic mineralization was detected in some samples at week 8 but there was no difference among different groups. The GFP-TDSC-Scx group only statistically significantly improved tendon repair histologically and biomechanically compared to the Scaffold-only group and the GFP-TDSC-Mock group at the early stage of tendon repair. There was significant higher expression of collagen type I in the window wound in the GFP-TDSC-Scx group compared to the other two groups at week 2. The transplantation of GFP-TDSC-Scx promoted healing at the early stage of tendon repair in a rat patellar tendon window injury model.
机译：我们假设，与模拟转导细胞的移植相比，Scx转导的肌腱干细胞（TDSC）的移植促进了更好的肌腱修复。因此，本研究旨在研究Scx转导对TDSC中谱系标志物表达的影响以及所得细胞系在促进肌腱修复中的作用。用Scx或空的慢病毒载体（Mock）转导大鼠非GFP或GFP-TDSC，并通过杀稻瘟菌素筛选。通过qRT-PCR检测Scx和不同谱系标记的mRNA表达。然后在大鼠单侧pa骨肌腱窗损伤模型中测试了GFP-TDSC-Scx移植对肌腱修复的影响。 GFP-TDSC-模拟物和仅支架的移植用作对照。在移植后第2、4和8周，收集修复的pa骨肌腱用于离体荧光成像，vivaCT成像，组织学，免疫组织化学和生物力学测试。与GFP-TDSC-Mock相比，GFP-TDSC-Scx始终显示出大多数与腱和软骨相关的标志物更高的表达。然而，Scx转导对骨相关标志物表达的影响尚无定论。移植的GFP-TDSCs在第2周时可在窗伤口中检出，但在第4周时未检出。在第8周时某些样品中检测到异位矿化，但不同组之间无差异。与仅支架的组和GFP-TDSC-Mock组相比，GFP-TDSC-Scx组在组织学和生物力学上仅在统计学上显着改善了肌腱修复的组织学和生物力学。在第2周时，GFP-TDSC-Scx组的窗口伤口中的I型胶原蛋白的表达明显高于其他两组。在第2周，GFP-TDSC-Scx的移植促进了肌腱修复早期的愈合。大鼠pa肌腱窗损伤模型

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