• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia
【2h】

Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia

机译：埃塞俄比亚远郊牧区肺结核患者结核分枝杆菌的菌株多样性

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Data on genotypic diversity of Mycobacterium tuberculosis complex (MTBC) is important to understand its epidemiology, human adaptation, clinical phenotypes, and drug resistance. This study aimed to characterize MTBC clinical isolates circulating in a predominantly pastoralist area in Ethiopia, a country where tuberculosis is the second leading cause of mortality. Culture of sputum samples collected from a total of 325 pulmonary TB suspects was done to isolate MTBC. Spoligotyping was used to characterize 105 isolates from culture positive slopes and the result was compared with an international database. Forty-four spoligotype patterns were observed to correspond to 35 shared-types (SITs) containing 96 isolates and 9 orphan patterns; 27 SITs containing 83 isolates matched a preexisting shared-type in the database, whereas 8 SITs (n = 13 isolates) were newly created. A total of 19 SITs containing 80 isolates were clustered within this study (overall clustering of 76.19%). Three dominant lineages (T, CAS, and Manu) accounted for 76.19% of the isolates. SIT149/T3-ETH was one of the two most dominant sublineages. Unlike previous reports, we show that Manu lineage strains not only constitute a dominant lineage, but are also associated with HIV infection in Afar region of Ethiopia. The high level of clustering suggests the presence of recent transmission that should be further studied using additional genotyping markers.
机译：关于结核分枝杆菌复合体（MTBC）基因型多样性的数据对于了解其流行病学，人类适应性，临床表型和耐药性具有重要意义。这项研究的目的是鉴定在埃塞俄比亚一个以牧民为主的地区中传播的MTBC临床分离株的特征，在埃塞俄比亚，结核病是造成死亡的第二大原因。从总共325名肺结核疑似患者中收集痰标本进行培养，以分离MTBC。寡核苷酸分型被用来表征来自文化阳性斜坡的105个分离株，并将结果与​​国际数据库进行比较。观察到44种spoligotype模式对应于35个共享类型（SIT），其中包含96个分离株和9个孤儿模式；包含83个分离株的27个SIT与数据库中预先存在的共享类型匹配，而新创建了8个SIT（n = 13个分离株）。这项研究共收集了19个包含80个分离株的SIT（总体聚集率为76.19％）。三个优势谱系（T，CAS和Manu）占分离株的76.19％。 SIT149 / T3-ETH是两个最主要的子系之一。与以前的报告不同，我们表明，Manu谱系不仅构成主要谱系，而且还与埃塞俄比亚Afar地区的HIV感染有关。高聚类表明存在近期传播，应使用其他基因分型标记物进一步研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号