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A subset of chicken statoacoustic ganglion neurites are repelled by Slit1 and Slit2

机译：Slit1和Slit2排斥了一部分鸡的听觉神经节神经突

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Mechanosensory hair cells in the chicken inner ear are innervated by bipolar afferent neurons of the statoacoustic ganglion (SAG). During development, individual SAG neurons project their peripheral process to only one of eight distinct sensory organs. These neuronal subtypes may respond differently to guidance cues as they explore the periphery in search of their target. Previous gene expression data suggested that Slit repellants might channel SAG neurites into the sensory primordia, based on the presence of robo transcripts in the neurons and the confinement of slit transcripts to the flanks of the prosensory domains. This led to the prediction that excess Slit proteins would impede the outgrowth of SAG neurites. As predicted, axonal projections to the primordium of the anterior crista were reduced 2-3 days after electroporation of either slit1 or slit2 expression plasmids into the anterior pole of the otocyst on embryonic day 3 (E3). The posterior crista afferents, which normally grow through and adjacent to slit expression domains as they are navigating towards the posterior pole of the otocyst, did not show Slit responsiveness when similarly challenged by ectopic delivery of slit to their targets. The sensitivity to ectopic Slits shown by the anterior crista afferents was more the exception than the rule: responsiveness to Slits was not observed when the entire E4 SAG was challenged with Slits for 40 hours in vitro. The corona of neurites emanating from SAG explants was unaffected by the presence of purified human Slit1 and Slit2 in the culture medium. Reduced axon outgrowth from E8 olfactory bulbs cultured under similar conditions for 24 hours confirmed bioactivity of purified human Slits on chicken neurons. In summary, differential sensitivity to Slit repellents may influence the directional outgrowth of otic axons toward either the anterior or posterior otocyst.
机译：鸡内耳的机械感觉毛细胞被静听神经节（SAG）的双极传入神经支配。在发育过程中，各个SAG神经元仅将其周围过程投射到八个不同的感觉器官之一。这些神经元亚型在探索周围区域以寻找靶标时，对指导线索的反应可能有所不同。先前的基因表达数据表明，基于神经元中存在机器人转录本并将狭缝转录本限制在前感觉域的侧面，Slit驱避剂可能会将SAG神经突引导到感觉原基中。这导致了以下预测：过量的Slit蛋白会阻碍SAG神经突的生长。如所预测的，在第3天（E3）将slit1或slit2表达质粒电穿孔入耳囊的前极后2-3天，到前cr的原基的轴突投影减少。当cr裂向耳囊后极导航时，通常在裂隙表达域附近生长并通过裂隙表达域生长的后cr突入孔，当类似地受到裂隙向其靶的异位递送的挑战时，并未显示裂隙反应性。前cr入者对异位狭缝的敏感性比一般情况更为例外：在体外用狭缝挑战整个E4 SAG 40小时后，未观察到对狭缝的反应性。 SAG外植体发出的神经突的电晕不受培养基中存在纯化的人Slit1和Slit2的影响。在类似条件下培养24小时的E8嗅球产生的轴突生长减少，这证实了纯化的人缝在鸡神经元上的生物活性。总而言之，对狭缝驱蚊剂的不同敏感性可能会影响耳轴突朝向前耳囊或后耳囊的方向性生长。

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