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首页> 美国卫生研究院文献>The Journal of Experimental Medicine >Restricted Immunoglobulin Variable Region (Ig V) Gene Expression Accompanies Secondary Rearrangements of Light Chain Ig V Genes in Mouse Plasmacytomas
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Restricted Immunoglobulin Variable Region (Ig V) Gene Expression Accompanies Secondary Rearrangements of Light Chain Ig V Genes in Mouse Plasmacytomas

机译：限制的免疫球蛋白可变区（Ig V）基因表达伴随小鼠浆细胞瘤中轻链Ig V基因的二次重排。

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The many binding studies of monoclonal immunoglobulin (Ig) produced by plasmacytomas have found no universally common binding properties, but instead, groups of plasmacytomas with specific antigen-binding activities to haptens such as phosphorylcholine, dextrans, fructofuranans, or dinitrophenyl. Subsequently, it was found that plasmacytomas with similar binding chain specificities not only expressed the same idiotype, but rearranged the same light (VL) and heavy (VH) variable region genes to express a characteristic monoclonal antibody. In this study, we have examined by enzyme-linked immunosorbent assay five antibodies secreted by silicone-induced mouse plasmacytomas using a broader panel of antigens including actin, myosin, tubulin, single-stranded DNA, and double-stranded DNA. We have determined the Ig heavy and light chain V gene usage in these same plasmacytomas at the DNA and RNA level. Our studies reveal: (a) antibodies secreted by plasmacytomas bind to different antigens in a manner similar to that observed for natural autoantibodies; (b) the expressed Ig heavy genes are restricted in V gene usage to the VH-J558 family; and (c) secondary rearrangements occur at the light chain level with at least three plasmacytomas expressing both κ and λ light chain genes. These results suggest that plasmacytomas use a restricted population of B cells that may still be undergoing rearrangement, thereby bypassing the allelic exclusion normally associated with expression of antibody genes.
机译：由浆细胞瘤产生的单克隆免疫球蛋白（Ig）的许多结合研究均未发现普遍通用的结合特性，而是具有对半抗原具有特定抗原结合活性的浆细胞瘤组，例如磷酰胆碱，葡聚糖，果糖呋喃或二硝基苯基。随后，发现具有相似结合链特异性的浆细胞瘤不仅表达相同的独特型，而且重新排列相同的轻（VL）和重（VH）可变区基因以表达特征性单克隆抗体。在这项研究中，我们通过酶联免疫吸附法检测了由硅酮诱导的小鼠浆细胞瘤分泌的五种抗体，这些抗体使用了范围更广的抗原，包括肌动蛋白，肌球蛋白，微管蛋白，单链DNA和双链DNA。我们已经确定了这些相同的浆细胞瘤在DNA和RNA水平上Ig重链和轻链V基因的用法。我们的研究表明：（a）浆细胞瘤分泌的抗体以与天然自身抗体相似的方式与不同抗原结合； （b）表达的Ig重基因在V基因使用中限于VH-J558家族； （c）次级重排发生在轻链水平，至少三个浆细胞瘤同时表达κ和λ轻链基因。这些结果表明浆细胞瘤使用的B细胞数量有限，可能仍在进行重排，从而绕过了通常与抗体基因表达相关的等位基因排斥。

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