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Effect of Glutaraldehyde Fixation on the Frictional Response of Immature Bovine Articular Cartilage Explants

机译：戊二醛固定对未成熟牛关节软骨外植体摩擦反应的影响

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This study examined functional properties and biocompatibility of glutaraldehyde-fixed bovine articular cartilage over several weeks of incubation at body temperature to investigate its potential use as a resurfacing material in joint arthroplasty. In the first experiment, treated cartilage disks were fixed using 0.02, 0.20 and 0.60 percent glutaraldehyde for 24 h then incubated, along with an untreated control group, in saline for up to 28 days at 37 °C. Both the equilibrium compressive and tensile moduli increased nearly twofold in treated samples compared to day 0 control, and remained at that level from day 1 to 28; the equilibrium friction coefficient against glass rose nearly twofold immediately after fixation (day 1) but returned to control values after day 7. Live explants co-cultured with fixed explants showed no quantitative difference in cell viability over 28 days. In general, no significant differences were observed between 0.20 and 0.60 percent groups, so 0.20 percent was deemed sufficient for complete fixation. In the second experiment, cartilage-on-cartilage frictional measurements were performed under a migrating contact configuration. In the treated group, one explant was fixed using 0.20 percent glutaraldehyde while the apposing explant was left untreated; in the control group both explants were left untreated. From day 1 to 28, the treated group exhibited either no significant difference or slightly lower friction coefficient than the untreated group. These results suggest that a properly titrated glutaraldehyde treatment can reproduce the desired functional properties of native articular cartilage and maintain these properties for at least 28 days at body temperature.
机译：这项研究检查了戊二醛固定的牛关节软骨在体温下培养数周后的功能特性和生物相容性，以研究其在关节置换术中作为铺面材料的潜在用途。在第一个实验中，使用0.02％，0.20％和0.60％的戊二醛将处理过的软骨盘固定24小时，然后与未处理的对照组一起在盐水中于37°C孵育28天。与第0天对照相比，处理后样品的平衡压缩模量和拉伸模量都增加了近两倍，并且从第1天到第28天一直保持在该水平。固定后（第1天），与玻璃的平衡摩擦系数几乎增加了两倍，但在第7天后恢复到对照值。与固定外植体共同培养的活外植体在28天中未显示出细胞活力的定量差异。通常，在0.20％和0.60％的组之间没有观察到显着差异，因此，将0.20％认为足以完全固定。在第二个实验中，在迁移接触结构下进行了软骨间摩擦力测量。在治疗组中，使用0.20％的戊二醛固定1个外植体，而未对置的外植体进行治疗。在对照组中，两种外植体都未经处理。从第1天到第28天，治疗组与未治疗组相比无明显差异或摩擦系数略低。这些结果表明，适当滴定的戊二醛治疗可以重现天然关节软骨的所需功能特性，并在体温下将这些特性保持至少28天。

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