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首页> 美国卫生研究院文献>other >First Nationwide Surveillance of Culex pipiens Complex and Culex torrentium Mosquitoes Demonstrated the Presence of Culex pipiens Biotype pipiens/molestus Hybrids in Germany
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First Nationwide Surveillance of Culex pipiens Complex and Culex torrentium Mosquitoes Demonstrated the Presence of Culex pipiens Biotype pipiens/molestus Hybrids in Germany

机译：首次在全国范围内对淡色库蚊和库蚊洪灾进行了监测证明了淡色库蚊/生物型蚊子在德国的存在。

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Mosquitoes and other arthropods may transmit medically important pathogens, in particular viruses such as West Nile virus. The presence of suitable hosts and competent vectors for those zoonotic viruses is essential for an enzootic transmission, which is a prerequisite for epidemics. To establish reliable risk projections, it is an urgent need for an exact identification of mosquito species, which is especially challenging in the case of sibling species, such as Culex. pipiens pipiens biotypes pipiens and molestus. To facilitate detection of different Culex pipiens forms and their hybrids we established a multiplex real-time PCR. Culex pipiens samples were obtained by egg raft collection and rearing until imago stage or adult sampling using CO2 baited traps and gravid traps. In total, we tested more than 16,500 samples collected all over Germany in the years 2011 and 2012. The predominant species in Germany are Culex pipiens pipiens biotype pipiens and Culex. torrentium, but we also detected Culex pipiens pipiens biotype molestus and hybrids of the two pipiens biotypes at sites where both species occur sympatrically. This report of a potentially important bridge vector for West Nile virus might have major impact in the risk projections for West Nile virus in Germany.
机译：蚊子和其他节肢动物可能传播医学上重要的病原体，尤其是病毒，例如西尼罗河病毒。对于那些人畜共患病毒而言，合适的宿主和有效载体的存在对于动物传播是必不可少的，这是流行的前提。为了建立可靠的风险预测，迫切需要准确识别蚊子物种，这对于同胞物种（例如库蚊）尤其具有挑战性。 pipiens pipiens生物型pipiens和molestus。为了便于检测不同的淡色库蚊形式及其杂种，我们建立了多重实时PCR。通过使用蛋排捕集器和妊娠诱集器通过卵筏收集和饲养直至成虫阶段或成年采样来获得淡色库蚊样品。在2011年和2012年期间，我们总共测试了超过16,500个样品，这些样品在德国全国范围内进行。德国的主要物种是pipiens pipiens pipiens生物型pipiens和Culex。洪流，但我们还发现了库蚊（Pilexens pipiens）pipiens生物型mole鼠和两种pipiens生物型的杂种在两个物种同时出现的地点。这份有关西尼罗河病毒潜在重要桥梁载体的报告可能会对德国的西尼罗河病毒风险预测产生重大影响。

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