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Antimicrobial Potential of Endophytic Fungi Derived from Three Seagrass Species: Cymodocea serrulata Halophila ovalis and Thalassia hemprichii

机译:来自三种海草物种的内生真菌的抗菌潜能:Cymodocea serrulata嗜盐菌 卵圆形和丘疹 大麻

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摘要

Endophytic fungi from three commonly found seagrasses in southern Thailand were explored for their ability to produce antimicrobial metabolites. One hundred and sixty endophytic fungi derived from Cymodocea serrulata (Family Cymodoceaceae), Halophila ovalis and Thalassia hemprichii (Family Hydrocharitaceae) were screened for production of antimicrobial compounds by a colorimetric broth microdilution test against ten human pathogenic microorganisms including Staphylococcus aureus ATCC 25923, a clinical isolate of methicillin-resistant S. aureus, Escherichia coli ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 90028 and NCPF 3153, Cryptococcus neoformans ATCC 90112 and ATCC 90113 and clinical isolates of Microsporumgypseum and Penicilliummarneffei. Sixty-nine percent of the isolates exhibited antimicrobial activity against at least one test strain. Antifungal activity was more pronounced than antibacterial activity. Among the active fungi, seven isolates including Hypocreales sp. PSU-ES26 from C. serrulata, Trichoderma spp. PSU-ES8 and PSU-ES38 from H. ovalis, and Penicillium sp. PSU-ES43, Fusarium sp. PSU-ES73, Stephanonectria sp. PSU-ES172 and an unidentified endophyte PSU-ES190 from T. hemprichii exhibited strong antimicrobial activity against human pathogens with minimum inhibitory concentrations (MIC) of less than 10 µg/ml. The inhibitory extracts at concentrations of 4 times their MIC destroyed the targeted cells as observed by scanning electron microscopy. These results showed the antimicrobial potential of extracts from endophytic fungi from seagrasses.
机译:研究了泰国南部三种常见海草的内生真菌产生抗菌代谢物的能力。通过比色肉汤微量稀释试验,对来自Symodocea serrulata(Cymodoceaceae),Halophila卵形和Thalsiasia hemprichii(Family Hydrocharitaceae )的一百六十种内生真菌进行了筛选。十种人类致病微生物,包括 金黄色葡萄球菌 ATCC 25923,这是耐甲氧西林的 S。金黄色葡萄球菌 大肠杆菌 ATCC 25923, 假单胞菌铜绿 ATCC 27853, 白色念珠菌 ATCC 90028和NCPF 3153, 新隐球菌 ATCC 90112和ATCC 90113以及 小孢子菌 石膏 青霉菌 marneffei 。 69%的分离株对至少一种测试菌株表现出抗菌活性。抗真菌活性比抗菌活性更明显。在活性真菌中,有七个分离株,包括 Hypocreales sp。来自的PSU-ES26 C serrulata 木霉 spp。 中的PSU-ES8和PSU-ES38 H 卵子 青霉菌 sp。 PSU-ES43, Fusarium sp。 PSU-ES73, Stephanonectria sp。 PSU-ES172和来自的身份不明的内生菌PSU-ES190 T Hemprichii 对人类病原体表现出强大的抗菌活性,最低抑菌浓度(MIC)小于10 µg / ml。通过扫描电子显微镜观察,抑制性提取物的MIC浓度是其MIC的4倍,破坏了靶细胞。这些结果显示了海草内生真菌提取物的抗菌潜力。

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