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首页> 美国卫生研究院文献>other >Novel genetic diversity within Anopheles punctimacula s.l.: Phylogenetic discrepancy between the Barcode cytochrome c oxidase I (COI) gene and the rDNA second internal transcribed spacer (ITS2)
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Novel genetic diversity within Anopheles punctimacula s.l.: Phylogenetic discrepancy between the Barcode cytochrome c oxidase I (COI) gene and the rDNA second internal transcribed spacer (ITS2)

机译：点状按蚊的新遗传多样性：条码细胞色素C氧化酶I（COI）基因与rDNA第二个内部转录间隔区（ITS2）之间的系统发育差异

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Anopheles punctimacula s.l. is a regional malaria vector in parts of Central America, but its role in transmission is controversial due to its unresolved taxonomic status. Two cryptic species, An. malefactor and An. calderoni, have been previously confused with this taxon, and evidence for further genetic differentiation has been proposed. In the present study we collected and morphologically identified adult female mosquitoes of An. punctimacula s.l. from 10 localities across Panama and one in Costa Rica. DNA sequences from three molecular regions, the three prime end of the mitochondrial cytochrome c oxidase I gene (3´ COI), the Barcode region in the five prime end of the COI (5´ COI), and the rDNA second internal transcribed spacer (ITS2) were used to test the hypothesis of new molecular lineages within An. punctimacula s.l. Phylogenetic analyses using the 3´ COI depicted six highly supported molecular lineages (A–F), none of which was An. malefactor. In contrast, phylogenetic inference with the 5´ COI demonstrated paraphyly. Tree topologies based on the combined COI regions and ITS2 sequence data supported the same six lineages as the 3´ COI alone. As a whole this evidence suggests that An. punctimacula s.l. comprises two geographically isolated lineages, but it is not clear whether these are true species. The phylogenetic structure of the An. punctimacula cluster as well as that of other unknown lineages (C type I vs C type II; D vs E) appears to be driven by geographic partition, because members of these assemblages did not overlap spatially. We report An. malefactor for the first time in Costa Rica, but our data do not support the presence of An. calderoni in Panama.
机译：点状按蚊是中美洲部分地区的疟疾传播媒介，但由于其尚未解决的生物分类地位，其在传播中的作用引起争议。两种隐性物种男性因素和安。卡尔德罗尼（Calderoni）以前已与该分类单元混淆，并提出了进一步遗传分化的证据。在本研究中，我们收集了An的成年雌性蚊子，并在形态上进行了鉴定。泪点s.l.来自巴拿马各地的10个地区和哥斯达黎加的1个地区。来自三个分子区域的DNA序列：线粒体细胞色素C氧化酶I基因的三个主要末端（3´COI），COI五个主要末端的条形码区域（5´COI）和rDNA第二个内部转录间隔区（ ITS2）被用来检验An内新分子谱系的假设。泪点s.l.使用3´COI进行的系统进化分析显示了6个高度支持的分子谱系（AF），其中一个都不是An。男性因素。相比之下，对5´COI的系统发育推断证明是近自然的。基于合并的COI区域和ITS2序列数据的树形拓扑支持与单独3´COI相同的六个谱系。总体而言，该证据表明An。泪点s.l.由两个地理上孤立的血统组成，但尚不清楚它们是否是真实物种。 An的系统发育结构。点状点簇以及其他未知谱系（C型I vs C型II； D vs E）似乎是由地理分区驱动的，因为这些组合的成员在空间上没有重叠。我们报告一个。哥斯达黎加首次出现“ malefactor” ，但我们的数据不支持 An的存在。巴拿马的calderoni 。

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