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Small GTPase Rab40c Associates with Lipid Droplets and Modulates the Biogenesis of Lipid Droplets

机译：小GTPase Rab40c与脂质液滴结合并调节脂质液滴的生物发生。

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The subcellular location and cell biological function of small GTPase Rab40c in mammalian cells have not been investigated in detail. In this study, we demonstrated that the exogenously expressed GFP-Rab40c associates with lipid droplets marked by neutral lipid specific dye Oil red or Nile red, but not with the Golgi or endosomal markers. Further examination demonstrated that Rab40c is also associated with ERGIC-53 containing structures, especially under the serum starvation condition. Rab40c is increasingly recruited to the surface of lipid droplets during lipid droplets formation and maturation in HepG2 cells. Rab40c knockdown moderately decreases the size of lipid droplets, suggesting that Rab40c is involved in the biogenesis of lipid droplets. Stimulation for adipocyte differentiation increases the expression of Rab40c in 3T3-L1 cells. Rab40c interacts with TIP47, and is appositionally associated with TIP47-labeled lipid droplets. In addition, over-expression of Rab40c causes the clustering of lipid droplets independent of its GTPase activity, but completely dependent of the intact SOCS box domain of Rab40c. In addition, Rab40c displayed self-interaction as well as interaction with TIP47 and the SOCS box is essential for its ability to induce clustering of lipid droplets. Our results suggest that Rab40c is a novel Rab protein associated with lipid droplets, and is likely involved in modulating the biogenesis of lipid droplets.
机译：尚未详细研究小型GTPase Rab40c在哺乳动物细胞中的亚细胞定位和细胞生物学功能。在这项研究中，我们证明了外源表达的GFP-Rab40c与以中性脂质特异性染料油红或尼罗红标记的脂滴相关，但与高尔基体或内体标记无关。进一步的检查表明，Rab40c也与含有ERGIC-53的结构有关，特别是在血清饥饿的情况下。 Rab40c在HepG2细胞中的脂滴形成和成熟过程中越来越多地募集到脂滴表面。 Rab40c敲低适度减少脂质滴的大小，表明Rab40c参与脂质滴的生物发生。刺激脂肪细胞分化可增加3T3-L1细胞中Rab40c的表达。 Rab40c与TIP47相互作用，并与TIP47标记的脂质滴并置。此外，Rab40c的过度表达会导致脂滴的聚集，而与其GTPase活性无关，但完全取决于Rab40c完整的SOCS盒结构域。另外，Rab40c表现出自我相互作用以及与TIP47的相互作用，而SOCS盒对于诱导脂质小滴聚集的能力至关重要。我们的结果表明，Rab40c是与脂滴相关的新型Rab蛋白，可能与调节脂滴的生物发生有关。

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