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首页> 美国卫生研究院文献>other >A nuclear single-nucleotide polymorphism (SNP) potentially useful for the separation of Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae)
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A nuclear single-nucleotide polymorphism (SNP) potentially useful for the separation of Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae)

机译：核单核苷酸多态性（SNP）潜在可用于分离rhodnius rubustus隐秘物种复合物的成员（Hemiptera：Reduviidae）的rhodnius咯

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The design and application of rational strategies that rely on accurate species identification are pivotal for effective vector control. When morphological identification of the target vector species is impractical, the use of molecular markers is required. Here we describe a non-coding, single-copy nuclear DNA fragment that contains a single-nucleotide polymorphism (SNP) with the potential to distinguish the important domestic Chagas disease vector, Rhodnius prolixus, from members of the four sylvatic Rhodnius robustus cryptic species complex. A total of 96 primer pairs obtained from whole genome shotgun sequencing of the R. prolixus genome (12,626 random reads) were tested on 43 R. prolixus and R. robustus s.l. samples. One of the seven amplicons selected (AmpG) presented a SNP, potentially diagnostic for R. prolixus, on the 280th site. The diagnostic nature of this SNP was then performed on 154 R. prolixus and R. robustus s.l. samples aimed at achieving the widest possible geographic coverage. The results of a 60% majority rule Bayesian consensus tree and a median-joining network constructed based on the genetic variability observed reveal the paraphyletic nature of the R. robustus species complex, with respect to R. prolixus. AmpG region is located in the fourth intron of the Transmembrane protein 165 gene, which seems to be in the R. prolixus X chromosome. Other possible chromosomal locations of the AmpG region in the R. prolixus genome are also presented and discussed.
机译：依靠准确的物种识别的合理策略的设计和应用对于有效的矢量控制至关重要。当目标载体物种的形态学鉴定不切实际时，则需要使用分子标记。在这里，我们描述了一个非编码，单拷贝的核DNA片段，其中包含一个单核苷酸多态性（SNP），有可能将重要的南美锥虫病载体Rhodnius prolixus与四个斯氏囊性Rhodniusrobustus隐性物种复合体的成员区分开。在43个Prolixus和R.robustus s.l.上测试了从Pro.prolixus基因组的全基因组shot弹枪测序获得的总共96对引物对（12,626个随机读数）。样品。选定的七个扩增子之一（AmpG）在第280个位点上显示了SNP，可能诊断为螺旋藻。然后在154个螺旋螺和鲁棒螺旋杆菌s.l.上进行该SNP的诊断性质。旨在实现尽可能广泛的地理覆盖范围的样本。 60％多数规则贝叶斯共识树和基于观察到的遗传变异性构建的中值连接网络的结果揭示了鲁棒罗非鱼种复合体相对于螺旋。的寄生虫学性质。 AmpG区位于跨膜蛋白165基因的第四个内含子中，该基因似乎位于R. prolixus X染色体中。还提出并讨论了螺旋藻基因组中AmpG区的其他可能的染色体位置。

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