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Patterns of Fluctuating Asymmetry and Shape Variation in Chironomus riparius (Diptera Chironomidae) Exposed to Nonylphenol or Lead

机译:波动对称的图案和形状(双翅目摇蚊)变异摇riparius暴露于壬基酚或铅

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摘要

Deformities and fluctuating asymmetry in chironomid larvae have been proposed as sensitive indicators of biological stress and are commonly used to assess the ecological impact of human activities. In particular, they have been associated in Chironomus riparius, the most commonly used species, with heavy metal and pesticide river pollution. In this study, the effect of lead and 4-nonylphenol on mouthpart morphological variation of Chironomus riparius larvae was investigated by traditional and geometric morphometrics. For this purpose, first to fourth instar larvae were exposed to sediment spiked with lead (from 3.0 to 456.9 mg/kg dry weight) or 4-NP (from 0.1 to 198.8 mg/kg dry weight). Mentum phenotypic response to pollutants was assessed by four parameters: (1) the frequency of deformities, (2) fluctuating asymmetry of mentum length, (3) fluctuating asymmetry of mentum shape and (4) the mentum mean shape changes. Despite the bioaccumulation of pollutants in the chironomid’s body, no significant differences between control and stressed groups were found for mouthpart deformities and fluctuating asymmetry of mentum length. Slight effects on mentum shape fluctuating asymmetry were observed for two stressed groups. Significant mean shape changes, consisting of tooth size increase and tooth closing, were detected for lead and 4-NP exposure respectively. Those variations, however, were negligible in comparison to mentum shape changes due to genetic effects. These results suggest that the use of mentum variation as an indicator of toxic stress in Chironomus riparius should be considered cautiously.
机译:已提出将拟虫幼虫的畸形和波动性不对称性作为生物胁迫的敏感指标,通常用于评估人类活动对生态的影响。特别是,它们已在最普遍使用的物种Chironomus riparius中与重金属和农药河污染有关。在这项研究中,通过传统的和几何形态学方法研究了铅和4-壬基苯酚对河岸线虫幼虫口部形态变化的影响。为此,将第一至第四龄幼虫暴露于掺有铅(从3.0至456.9 mg / kg干重)或4-NP(从0.1至198.8 mg / kg干重)的沉淀物中。通过四个参数评估心理行为对污染物的表型反应:(1)畸变的频率;(2)长度的波动不对称;(3)shape形状的波动不对称;(4)mean平均形状的变化。尽管手足动物体内有污染物的生物蓄积,但对照组和压力组在口器畸形和精神错乱长度的不对称性上没有发现显着差异。在两个压力组中观察到对脑膜形状波动不对称性的轻微影响。分别针对铅和4-NP暴露检测到显着的平均形状变化,包括牙齿大小增加和牙齿闭合。然而,与由于遗传效应引起的精神形态改变相比,这些变化可以忽略不计。这些结果表明,应谨慎考虑使用口气变化作为滨藜(Chironomus riparius)中毒性应激的指标。

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