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The Temperature Dependent Proteomic Analysis of Thermotoga maritima

机译：温度喜温海洋的依赖蛋白质组学分析

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Thermotoga maritima (T. maritima) is a typical thermophile, and its proteome response to environmental temperature changes has yet to be explored. This study aims to uncover the temperature-dependent proteins of T. maritima using comparative proteomic approach. T. maritima was cultured under four temperatures, 60°C, 70°C, 80°C and 90°C, and the bacterial proteins were extracted and electrophoresed in two-dimensional mode. After analysis of gel images, a total of 224 spots, either cytoplasm or membrane, were defined as temperature-dependent. Of these spots, 75 unique bacterial proteins were identified using MALDI TOF/TOF MS. As is well known, the chaperone proteins such as heat shock protein 60 and elongation factor Tu, were up-regulated in abundance due to increased temperature. However, several temperature-dependent proteins of T. maritima responded very differently when compared to responses of the thermophile T. tengcongensis. Intriguingly, a number of proteins involved in central carbohydrate metabolism were significantly up-regulated at higher temperature. Their corresponding mRNA levels were elevated accordingly. The increase in abundance of several key enzymes indicates that a number of central carbohydrate metabolism pathways of T. maritima are activated at higher temperatures.
机译：maritoma（T. maritima）是典型的嗜热菌，其蛋白质组对环境温度变化的反应尚待探索。这项研究的目的是使用比较蛋白质组学方法来发现海螯虾的温度依赖性蛋白。在60°C，70°C，80°C和90°C的四种温度下培养海藻圆球菌，并提取细菌蛋白质并以二维模式进行电泳。分析凝胶图像后，总共224个斑点（细胞质或膜）被定义为温度依赖性。在这些斑点中，使用MALDI TOF / TOF MS鉴定出75种独特的细菌蛋白。众所周知，由于温度升高，伴侣蛋白如热激蛋白60和延伸因子Tu被大量上调。但是，与嗜热嗜热球菌T. congconensis的反应相比，几种嗜热球菌的温度依赖性蛋白的反应非常不同。有趣的是，在较高温度下，参与中央碳水化合物代谢的许多蛋白质均明显上调。它们相应的mRNA水平也相应升高。几种关键酶的丰度增加表明，在较高的温度下激活了海螯虾的许多主要碳水化合物代谢途径。

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