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Surface modification of plasmonic nanostructured materials with thiolated oligonucleotides in 10 seconds using selective microwave heating

机译:使用选择性微波加热在10秒内用硫化寡核苷酸的等离子体纳米结构材料的表面改性

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摘要

This study demonstrates the proof-of-principle of rapid surface modification of plasmonic nanostructured materials with oligonucleotides using low power microwave heating. Due to their interesting optical and electronic properties, silver nanoparticle films (SNFs, 2 nm thick) deposited onto glass slides were used as the model plasmonic nanostructured materials. Rapid surface modification of SNFs with oligonucleotides was carried out using two strategies (1) Strategy 1: for ss-oligonucleotides, surface hybridization and (2) Strategy 2: for ds-oligonucleotides, solution hybridization), where the samples were exposed to 10, 15, 30 and 60 seconds microwave heating. To assess the efficacy of our new rapid surface modification technique, identical experiments carried out without the microwave heating (i.e., conventional method), which requires 24 hours for the completion of the identical steps. It was found that SNFs can be modified with ss- and ds-oligonucleotides in 10 seconds, which typically requires several hours of incubation time for the chemisorption of thiol groups on to the planar metal surface using conventional techniques.
机译:这项研究证明了使用低功率微波加热用寡核苷酸对等离激元纳米结构材料进行快速表面修饰的原理证明。由于其有趣的光学和电子特性,沉积在载玻片上的银纳米颗粒薄膜(SNF,2 nm厚)被用作模型等离子体纳米结构材料。使用两种策略对寡核苷酸进行SNF的快速表面修饰(1)策略1:用于ss-寡核苷酸,表面杂交;(2)策略2:用于ds-寡核苷酸,溶液杂交),将样品暴露于10, 15、30和60秒的微波加热。为了评估我们新的快速表面改性技术的功效,在没有微波加热的情况下(即传统方法)进行了相同的实验,该过程需要24小时才能完成相同的步骤。发现SNF可以在10秒内用ss-和ds-寡核苷酸修饰,这通常需要数小时的温育时间以使用常规技术将硫醇基化学吸附到平面金属表面上。

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