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Pretreatment of whole blood using hydrogen peroxide and UV irradiation. Design of the advanced oxidation process

机译:使用过氧化氢和紫外线辐射进行全血的预处理。设计先进的氧化过程

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摘要

A new process to pretreat blood samples has been developed. This process combines the Advanced Oxidation Process (AOP) treatment (using H2O2 and UV irradiation) with acid deactivation of the enzyme catalase in blood. A four-cell reactor has been designed and built in house. The effect of pH on the AOP process has been investigated. The kinetics of the pretreatment process shows that at high CH2O2,t = 0, the reaction is zeroth order with respect to CH2O2 and first order with respect to Cblood. The rate limiting process is photon flux from the UV lamp. Degradation of whole blood has been compared with that of pure hemoglobin samples. The AOP pretreatment of the blood samples has led to the subsequent determination of chromium and zinc concentrations in the samples using electrochemical methods.
机译:已经开发出预填充血液样本的新方法。该方法结合了先进的氧化过程(AOP)处理(使用H2O2和UV辐射)与血液中酶过氧化酶的酸失活。四个细胞反应器已经设计和建造。研究了pH对AOP过程的影响。预处理方法的动力学表明,在高CH 2 O 2,T = 0时,反应是相对于CH 2 O 2和相对于Cblood的第一阶的zeroth阶。速率限制过程是来自UV灯的光子通量。将全血的降解与纯血红蛋白样品进行了比较。使用电化学方法,血液样品的AOP预处理导致了随后的样品中铬和锌浓度的测定。

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