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Soymilk: an effective and inexpensive blocking agent for immunoblotting

机译:豆浆:一种用于免疫印迹的有效且廉价的阻断剂

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摘要

Blocking efficacy of whole soymilk, non-fat soymilk, SuperBlock™, and non-fat milk was evaluated by performing standard protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293 cells. Non-fat soymilk was found to have superior blocking efficacy compared to other blocking agents in terms of high signal to noise ratio with the shortest blocking times. In addition, the presence of low concentrations of the detergent Tween-20 (0.05–0.1% v/v) in the wash buffer, as well as, antibody incubations significantly lessened the background compared to only including the detergent during wash steps.
机译:通过对纯化的蛋白质和来自HEK 293细胞的粗核提取物进行标准蛋白质免疫印迹程序来评估整个豆浆,非脂肪豆浆,超簇™和非脂肪乳的疗效。发现非脂肪豆浆与其他阻塞剂在高信号与最短阻塞时间的噪声比相比具有卓越的阻塞效果。此外,与仅在洗涤步骤中的洗涤剂包括洗涤剂相比,洗涤缓冲液中的低浓度的洗涤剂吐温-20(0.05-0.1%v / v)的存在显着减少了背景。

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