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首页> 美国卫生研究院文献>other >A portable cryo-plunger for on-site intact cryogenic microscopy sample preparation in natural environments
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A portable cryo-plunger for on-site intact cryogenic microscopy sample preparation in natural environments

机译：用于在现场的便携式冷冻柱塞完整的低温显微镜样品在自然环境中制备

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We present a modern, light portable device specifically designed for environmental samples for cryo-electron microscopy by on-site cryo-plunging. The power of cryogenic EM comes from preparation of artifact-free samples. However, in many studies the samples must be collected at remote field locations, and the time involved in transporting samples back to the laboratory for cryogenic preservation can lead to severe degradation artifacts. Thus, going back to the basics, we developed a simple mechanical device that is light and easy to transport on foot yet effective. With the system design presented here we are able to obtain cryo-samples of microbes and microbial communities not possible to culture, in their near-intact environmental conditions as well as in routine laboratory work, and in real time. This methodology thus enables us to bring the power of cryo-TEM to microbial ecology.

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