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Improving Liquid Chromatography-Mass Spectrometry Sensitivity Using a Subambient Pressure Ionization with Nanoelectrospray (SPIN) Interface

机译：利用纳米电子助推（旋转）界面的小除压力电离改善液相色谱 - 质谱敏感性

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摘要

In this work, the subambient pressure ionization with nanoelectrospray (SPIN) ion source and interface, which operates at ~15–30 Torr, is demonstrated to be compatible with gradient reversed-phase liquid chromatography-MS applications, exemplified here with the analysis of complex samples (a protein tryptic digest and a whole cell lysate). A low liquid chromatographic flow rate (100–400 nL/min) allowed stable electrospray to be established while avoiding electrical breakdown. Efforts to increase the operating pressure of the SPIN source relative to previously reported designs prevented solvent freezing and enhanced charged cluster/droplet desolvation. A 5- to 12-fold improvement in sensitivity relative to a conventional atmospheric pressure nanoelectrospray ionization (ESI) source was obtained for detected peptides.

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期刊名称 other
作者
Keqi Tang; Jason S. Page; Ioan Marginean; Ryan T. Kelly; Richard D. Smith;
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年(卷),期 -1(22),8
年度 -1
页码 1318–1325
总页数 14
原文格式 PDF
正文语种
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关键词
ESI LC-MS Sensitivity Subambient pressure ESI Ion source;

机译：ESI;LC-MS;灵敏度;环境压力ESI;离子源;

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专利

1. Improving liquid chromatography-mass spectrometry sensitivity using a subambient pressure ionization with nanoelectrospray (SPIN) interface [J] . Tang K., Page J.S., Marginean I., Journal of the American Society for Mass Spectrometry . 2011,第8期

机译：使用低压电离和纳米电喷雾（SPIN）界面提高液相色谱-质谱联用的灵敏度
2. Subambient pressure ionization with nanoelectrospray source and interface for improved sensitivity in mass spectrometry [J] . Page JS, Tang K, Kelly RT, Analytical chemistry . 2008,第5期

机译：具有纳米电喷雾源和界面的环境压力离子化技术可改善质谱的灵敏度
3. High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures [J] . Jonathan T. Cox, Scott R. Kronewitter, Anil K. Shukla, Analytical chemistry . 2014,第19期

机译：高感度结合不稳定化合物的结构覆盖范围，通过低于环境压力的纳米电喷雾电离
4. A New Ionization Source for Mass Spectrometry: Subambient Pressure Ionization with Nanoelectrospray (SPIN) [C] . Jason S. Page, Keqi Tang, Ryan T. Kelly, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：用于质谱的新电离源：用纳米电池（旋转）的脱节压力电离
5. Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives. [D] . Gao, Songmei. 2005

机译：使用衍生化和流动相添加剂在液相色谱/大气压电离质谱中提高灵敏度。
6. A subambient pressure ionization with nanoelectrospray (SPIN) source and interface for improved sensitivity in mass spectrometry [O] . Jason S. Page, Keqi Tang, Ryan T. Kelly, -1

机译：具有纳米电喷雾（SPIN）源和界面的低压电离可提高质谱的灵敏度
7. Subambient Pressure Ionization with Nanoelectrospray Source and Interface for Improved Sensitivity in Mass Spectrometry [O] . Jason S. Page, Keqi Tang, Ryan T. Kelly, 2008

机译：具有纳米电子涂布源和界面的脱模压力电离，用于提高质谱中的灵敏度

Improving Liquid Chromatography-Mass Spectrometry Sensitivity Using a Subambient Pressure Ionization with Nanoelectrospray (SPIN) Interface

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