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Transcriptome analysis of abscisic acid induced 20E regulation in suspension Ajuga lobata cells

机译：脱落酸诱导的悬浮朱ju细胞中20E调控的转录组分析

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摘要

Ajuga lobata D. Don is a medicinal plant rich in 20-hydroxyecdysone (20E), alkaloids, and other active substances. In this study, the cell suspension was incubated for 7 days, followed by the analysis on the effects of abscisic acid (ABA) on the regulation of 20E synthesis. Then A. lobata suspension cells treated with 0.15 mg/l ABA were used as material, with the Illumina technology applied for transcriptome sequencing. Digital analysis on the gene expression profile was carried out on ABA treated and control samples, respectively. Finally, transcriptomics was applied to assess the molecular response of A. lobata induced by ABA through applying transcriptomics by evaluating differentially expressed genes. The results suggested that ABA promoted 20E accumulation, while longer processing time caused cell browning. A total of 154 genes were significantly regulated after ABA treatment, with 99 up-regulated and 55 down-regulated, respectively. In addition to 20E-related pathways, the genes belonged to the ko00900 (terpenoid backbone biosynthesis) pathway (six differentially expressed genes [DEGs]), ko00100 (steroid biosynthesis) pathway (four DEGs), and ko00140 (steroid hormone biosynthesis) pathway (six DEGs). Providing a better understanding of the 20E biosynthetic pathway and its regulation, in particular in plants, this study is necessary.
机译：Ajuga lobata D. Don是富含20-羟基蜕皮激素（20E），生物碱和其他活性物质的药用植物。在这项研究中，将细胞悬液孵育7天，然后分析脱落酸（ABA）对20E合成调控的影响。然后将经0.15 mg / l ABA处理的球果悬浮细胞用作材料，并将Illumina技术应用于转录组测序。基因表达谱的数字分析分别在ABA处理的样品和对照样品上进行。最后，转录组学被用于通过评估差异表达的基因来应用转录组学来评估由ABA诱导的球叶农杆菌的分子反应。结果表明，ABA促进了20E的积累，而更长的处理时间导致了细胞褐变。 ABA处理后，共有154个基因被显着调节，分别有99个上调和55个下调。除了20E相关的途径外，这些基因还属于ko00900（萜类骨架生物合成）途径（六个差异表达基因[DEG]），ko00100（类固醇生物合成）途径（四个DEG）和ko00140（类固醇激素生物合成）途径（六个DEG）。为了更好地理解20E生物合成途径及其调控，特别是在植物中，这项研究是必要的。

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