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首页> 美国卫生研究院文献>PLoS Neglected Tropical Diseases >In vitro studies of Rickettsia-host cell interactions: Confocal laser scanning microscopy of Rickettsia helvetica-infected eukaryotic cell lines
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In vitro studies of Rickettsia-host cell interactions: Confocal laser scanning microscopy of Rickettsia helvetica-infected eukaryotic cell lines

机译：立克次体与宿主细胞相互作用的体外研究：共聚焦激光扫描显微镜观察感染立克次体的真核细胞系

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Rickettsia (R.) helvetica is the most prevalent rickettsia found in Ixodes ricinus ticks in Germany. Several studies reported antibodies against R. helvetica up to 12.5% in humans investigated, however, fulminant clinical cases are rare indicating a rather low pathogenicity compared to other rickettsiae. We investigated growth characteristics of R. helvetica isolate AS819 in two different eukaryotic cell lines with focus on ultra-structural changes of host cells during infection determined by confocal laser scanning microscopy. Further investigations included partially sequencing of rickA, sca4 and sca2 genes, which have been reported to encode proteins involved in cell-to-cell spread and virulence in some rickettsiae. R. helvetica grew constantly but slowly in both cell lines used. Confocal laser scanning microscopy revealed that the dissemination of R. helvetica AS819 in both cell lines was rather mediated by cell break-down and bacterial release than cell-to-cell spread. The cytoskeleton of both investigated eukaryotic cell lines was not altered. R. helvetica possesses rickA, but its expression is not sufficient to promote actin-based motility as demonstrated by confocal laser scanning microscopy. Hypothetical Sca2 and Sca4 proteins were deduced from nucleotide gene sequences but the predicted amino acid sequences were disrupted or truncated compared to other rickettsiae most likely resulting in non-functional proteins. Taken together, these results might give a first hint to the underlying causes of the reduced virulence and pathogenicity of R. helvetica.
机译：立克次体是德国最常见的立克次体。几项研究报道，在接受研究的人类中，抗黑头病菌的抗体高达12.5％，然而，暴发性临床病例很少，与其他立克次体相比，其致病性较低。我们研究了共聚焦激光扫描显微镜检测到的两种不同的真核细胞系中黑芥分离株AS819的生长特征，重点是宿主细胞在感染过程中的超微结构变化。进一步的研究包括rickA，sca4和sca2基因的部分测序，据报道这些基因编码某些立克次体中涉及细胞间扩散和致病性的蛋白质。 Helvetica R.在所用的两种细胞系中均不断生长，但生长缓慢。共聚焦激光扫描显微镜显示，两种细胞系中Helvetica AS819的传播是由细胞分解和细菌释放介导的，而不是细胞间的传播。两个研究的真核细胞系的细胞骨架均未改变。 R. helvetica 具有 rickA ，但共聚焦激光扫描显微镜证明其表达不足以促进基于肌动蛋白的运动。从核苷酸基因序列推定了假想的Sca2和Sca4蛋白，但是与其他立克次体相比，预测的氨基酸序列被打断或截断了，最有可能导致无功能的蛋白。综上所述，这些结果可能首先提示 R 的致病力和致病性降低的根本原因。 helvetica 。

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