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首页> 美国卫生研究院文献>other >Capillary Isoelectric Focusing Coupled Offline to Matrix Assisted Laser Desoprtion/Ionization Mass Spectrometry with Syringe Pump Mobilization
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Capillary Isoelectric Focusing Coupled Offline to Matrix Assisted Laser Desoprtion/Ionization Mass Spectrometry with Syringe Pump Mobilization

机译：毛细管等电聚焦与矩阵辅助激光脱水/电离质谱耦合用注射器泵动员耦合到基质辅助激光脱水/电离质谱

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This work presents several critical details for making cIEF-MALDI-MS a robust technique which will allow for more routine application and aid in automation. This includes emphasis on the hardware necessary for syringe pump mobilization and proper protocol for preventing disruption from gas bubbles. Following these guidelines, excellent elution time reproducibility is demonstrated for six pI markers (RSD < 5%). Additionally, the pI markers are used to calibrate the pH gradient and determine experimental pIs of proteins detected offline by mass spectrometry. This was demonstrated using a standard protein mixture of myoglobin and two forms of β-lactoglobulin. Experimental determination of protein pIs and molecular weights were found to be in agreement with literature values. The technical details discussed provide a sound foundation for applying the offline coupling of MALDI-MS with cIEF.

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