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Macromolecular scaffolds for immobilizing small molecule microarrays in label-free detection of protein-ligand interactions on solid support

机译：在固体载体上的蛋白质 - 配体相互作用的无标记检测固定小分子微阵列的大分子支架

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We explored two macromolecular scaffolds, bovine serum albumin (BSA) and polyvinyl alcohol (PVA), as chemically complementary platforms for immobilizing small molecule compounds on functionalized glass slides. We conjugated biotin molecules to BSA and amine-derivatized PVA and subsequently immobilized the conjugates on epoxy- functionalized glass slides through reaction of free amine residues on BSA and PVA with surface-bound epoxy groups. We studied binding reactions of such immobilized small molecule targets with solution-phase protein probes using an oblique-incidence reflectivity difference scanning optical microscope. The results showed that both BSA and amine-derivatized PVA were effective and efficient as carriers of small molecules with NHS residues and fluoric residues and for immobilization on epoxy-coated solid surfaces. A significant fraction of the conjugated small molecules retain their innate chemical activity.

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作者
Y. S. Sun; J. P. Landry; Y. Y. Fei; X. D. Zhu; J. T. Luo; X. B. Wang; K. S. Lam;
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年(卷),期 -1(81),13
年度 -1
页码 5373–5380
总页数 19
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1. Macromolecular Scaffolds for Immobilizing Small Molecule Microarrays in Label-Free Detection of Protein-Ligand Interactions on Solid Support [J] . Y. S. Sun, J. P. Landry, Y. Y. Fei, Analytical chemistry . 2009,第13期

机译：大分子支架固定小分子微阵列在固体支持物上的蛋白质-配体相互作用的无标记检测。
2. Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry [J] . Juergen Burger, Christin Rath, Johannes Woehrle, SLAS Technology . 2017,第4期

机译：成像反射式干涉仪的小批量无标记分子芯片上蛋白质相互作用的检测。
3. Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry: [J] . Juergen Burger, Christin Rath, Johannes Woehrle, Journal of Laboratory Automation . 2017,第4期

机译：成像反射式干涉仪的小批量无标记分子芯片上蛋白质相互作用的检测：
4. Label-free optical detection of small-molecule compound microarrays immobilized on solid support using macromolecular scaffolds and subsequent protein binding reactions [C] . Y.S. Sun, J.P. Landry, Y.Y. Fei, Imaging, manipulation, and analysis of biomolecules, cells, and tissues VII . 2009

机译：使用大分子支架和随后的蛋白结合反应对固定在固体支持物上的小分子化合物微阵列进行无标记光学检测
5. Label-free detection of biomolecular interactions in microarray format using oblique-incidence reflectivity difference microscopes [D] . Sun, Yung-Shin 2010

机译：使用斜发射反射差异显微镜无标记的微阵列形式的生物分子相互作用的检测
6. High Throughput Label-free Screening Small Molecule Compound Libraries for Protein-Ligands using Combination of Small Molecule Microarrays and a Special Ellipsometry-based Optical Scanner [O] . James P. Landry, Yiyan Fei, X.D. Zhu -1

机译：高吞吐量利用小分子微阵列和基于椭偏仪特殊光学扫描仪结合蛋白配体标记筛选小分子化合物库
7. Macromolecular Scaffolds for Immobilizing Small Molecule Microarrays in Label-Free Detection of Protein−Ligand Interactions on Solid Support [O] . Y. S. Sun, J. P. Landry, Y. Y. Fei, 2009

机译：用于固定小分子微阵列的大分子支架在固体载体上无标记的蛋白质 - 配体相互作用中的小分子微阵列

Macromolecular scaffolds for immobilizing small molecule microarrays in label-free detection of protein-ligand interactions on solid support

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