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QUANTITATIVE MEASUREMENT OF MULTIFUNCTIONAL QUANTUM DOT BINDING TO CELLULAR TARGETS USING FLOW CYTOMETRY

机译：流式细胞术多官能量子点与细胞靶标的定量测量

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摘要

BackgroundSemiconductor nanocrystals such as quantum dots (QDs) are a potentially powerful resource in the fields of flow cytometry and fluorescence microscopy. QD size and fluorescence characteristics offer attractive features for use in targeted delivery systems and detection by flow cytometry. While quantitative measurements of a variety of fluorescent molecules are routinely performed, fluorophores for which no calibration standards exist, such as QDs, pose a problem for quantitation in flow cytometry. Our goal was to develop a targeted nanoparticle delivery platform as well as a corresponding method to accurately and quantitatively assess the performance of this system.

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期刊名称 other
作者
RA Smith; TD Giorgio;
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年(卷),期 -1(75),5
年度 -1
页码 465–474
总页数 20
原文格式 PDF
正文语种
中图分类
关键词
flow cytometry fluorometry quantum dot nanocrystal quantitative calibration NGR PEG;

机译：流式细胞仪;荧光法;量子点;纳米晶体;定量;校准;NGR;PEG;

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专利

1. Quantitative Measurement of Multifunctional Quantum Dot Binding to Cellular Targets Using Flow Cytometry [J] . Smith RA, Giorgio TD Cytometry, Part A: the journal of the International Society for Analytical Cytology . 2009,第5期

机译：使用流式细胞仪定量测量多功能量子点与细胞靶标的结合
2. Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry [J] . Jun Wang, Yihong Zhan, Ning Bao Lab on a chip . 2012,第8期

机译：使用基于微流逝波的流式细胞仪在细胞群体水平上定量测量量子点摄取
3. Semi-quantitative Estimation of Cellular SiO_2 Nanoparticles Using Flow Cytometry Combined with X-ray Fluorescence Measurements [J] . Seo Yeon Choi, Nuri Yang, Soo Kyung Jeon, Cytometry, Part A: the journal of the International Society for Analytical Cytology . 2014,第9期

机译：流式细胞仪结合X射线荧光测量法对细胞SiO_2纳米颗粒的半定量估计
4. High-Throughput Measurement of the Long Excited-state Lifetime of Quantum Dots in Flow Cytometry [C] . Eshan Dahal, Ruofan Cao, Patrick Jenkins, Imaging, manipulation, and analysis of biomolecules, cells, and tissues XII . 2014

机译：流式细胞术中量子点长激发态寿命的高通量测量
5. Development of peptide targeted liposomes and in vitro assays for binding quantification and optimization under flow using microfluidics and flow cytometry [D] . Kusunose, Jiro 2013

机译：使用微流控和流式细胞仪开发靶向肽的脂质体并进行体外测定以进行结合定量和优化
6. Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements [O] . Rowena Mittal, Marcel P. Bruchez -1

机译：用于定量量子点测量的流式细胞仪的校准
7. Quantitative measurement of multifunctional quantum dot binding to cellular targets using flow cytometry [O] . R. A. Smith, T. D. Giorgio 2009

机译：流式细胞术多官能量子点与细胞靶标的定量测量

QUANTITATIVE MEASUREMENT OF MULTIFUNCTIONAL QUANTUM DOT BINDING TO CELLULAR TARGETS USING FLOW CYTOMETRY

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