首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Correlation studies of entero-set 20 API 20E and conventional media systems for Enterobacteriaceae identification.
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Correlation studies of entero-set 20 API 20E and conventional media systems for Enterobacteriaceae identification.

机译:肠道菌群20API 20E和常规培养基系统用于肠杆菌科鉴定的相关性研究。

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摘要

The Entero-Set kit (Fisher Diagnostics) is a 20-biochemical-test system used in the identification of members of the Enterobacteriaceae. This kit was compared with the API 20E (Analytab Products) and conventional media systems, using 505 (303 stock and 202 clinical) strains of Enterobacteriaceae. When the Entero-Set and API 20E results were compared with those of the conventional media system, the Entero-Set performed as well as the API 20E in overall identification. Comparison of common biochemical tests among the various systems showed that citrate, arabinose, adonitol, inositol, and malonate gave correlations below 90%. The majority of the discrepancies were found among stock cultures. In addition, most discrepancies occurred with species of Enterobacter, Salmonella, Proteus, Klebsiella, and Serratia. Reproducibility studies showed the Entero-Set system to perform with a high degree of accuracy and reproducibility.
机译:Entero-Set试剂盒(Fisher Diagnostics)是一种20生化测试系统,用于鉴定肠杆菌科的成员。使用505种肠杆菌科菌株(303种原种和202种临床菌株),将该试剂盒与API 20E(Analytab产品)和常规培养基系统进行了比较。当将Entero-Set和API 20E的结果与常规媒体系统的结果进行比较时,在整体识别方面,Entero-Set的表现与API 20E相同。各种系统之间常用生化测试的比较表明,柠檬酸盐,阿拉伯糖,腺苷,肌醇和丙二酸的相关性低于90%。大多数差异是在种群文化中发现的。此外,大多数差异发生于肠杆菌,沙门氏菌,变形杆菌,克雷伯菌和沙雷氏菌。再现性研究表明,Entero-Set系统可实现高度的准确性和再现性。

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