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m-Aminophenylboronic Acid as a Functional Monomer for Fabricating Molecularly Imprinted Polymer for the Recognition of Bovine Serum Albumin

机译：间氨基苯硼酸作为功能性单体用于制备分子印迹聚合物以识别牛血清白蛋白

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摘要

Using m-aminophenylboronic acid (APBA) as a functional monomer, molecularly imprinted polymer (MIP) imprinted with bovine serum albumin (BSA) was fabricated on activated glass spheres under optimized conditions. Key factors in the prepolymerization reaction (between APBA and BSA), such as buffer pH, ionic strength and reaction time, were carefully optimized as previously reported []. The interaction between APBA and BSA during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. Protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. The key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. Results demonstrated that the selectivity of MIP towards its template protein is superior to that of non-imprinted polymer (NIP).
机译：以间氨基苯基硼酸（APBA）为功能单体，在优化条件下，在活化玻璃球上制备了印迹有牛血清白蛋白（BSA）的分子印迹聚合物（MIP）。如先前报道的那样，精心优化了预聚合反应（APBA和BSA之间）中的关键因素，例如缓冲液的pH值，离子强度和反应时间。研究并优化了预聚合阶段APBA与BSA之间的相互作用，并确定了蛋白质重新结合实验的理想条件。分别在单一和竞争性结合方案中研究了聚合物的蛋白质重新结合和富集特性。本文的重点是可以通过从蛋白质饱和的聚合物中回收的结合蛋白的量来估算聚合物的结合选择性。结果表明，MIP对其模板蛋白的选择性优于非印迹聚合物（NIP）。

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