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Ligand and Protein Fishing with Heat Shock Protein 90 Coated Magnetic Beads

机译：带热激蛋白90涂层磁珠的配体和蛋白捕鱼

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Heat shock protein 90α (Hsp90α) is a molecular chaperone that has been targeted for the development of new anticancer therapies. To date, co-immunoprecipitation (IP) has been primarily used to identify novel client proteins. We now report an alternative approach in which Hsp90α has been immobilized onto the surface of silica-based magnetic beads. The beads were used to isolate known Hsp90α ligands from a mixture containing ligands and nonligands. In addition, they were also used to isolated proteins from a mixture of proteins, as well as a cellular extract. The results indicate that the Hsp90α coated magnetic beads can be used to “fish” from complex chemical and biological mixtures for new lead drug candidates and client proteins.
机译：热休克蛋白90α（Hsp90α）是一种分子伴侣，已被用于开发新的抗癌疗法。迄今为止，共免疫沉淀（IP）已主要用于鉴定新型客户蛋白。现在我们报告另一种方法，其中将Hsp90α固定在二氧化硅基磁珠的表面上。这些珠子用于从含有配体和非配体的混合物中分离出已知的Hsp90α配体。此外，它们还用于从蛋白质混合物以及细胞提取物中分离蛋白质。结果表明，Hsp90α包被的磁珠可用于从复杂的化学和生物混合物中“捕捞”新的候选候选药物和客户蛋白质。

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