首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.
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Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.

机译:通过细胞脂肪酸气液色谱自动鉴定细菌的最佳数据处理程序。

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摘要

Gas-liquid chromatography of cellular fatty acids was used in automatic identification of clinical bacterial isolates. The intraspecies variation in the occurrence of fatty acids and the variation in the relative gas-liquid chromatography peak areas of different fatty acids were evaluated and compared with the relative peak areas of these acids. A new chromatogram comparison method involving the use of an exponential function was developed to adjust to data variation optimally. This method was compared with several previously published methods of correlation analysis with data from representative clinical bacteriological isolates. The efficacies of the methods in separating different bacterial species into distinct clusters were compared. The new exponential function method was superior to the others both in its ability to separate species into different clusters and in giving a greater degree of identity to strains within a proper cluster. The results indicate that the gas-liquid chromatography of bacterial cellular fatty acids can be used effectively in the identification of clinically isolated bacteria. However, the usefulness of the analysis depends on the comparison method used and on its ability to cope with data variations.
机译:细胞脂肪酸的气液色谱法被用于自动鉴定临床细菌分离株。评价脂肪酸的种内变化和不同脂肪酸的相对气-液相色谱峰面积的变化,并将其与这些酸的相对峰面积进行比较。开发了一种新的色谱比较方法,该方法涉及使用指数函数,以最佳地适应数据变化。将该方法与几种先前发表的相关分析方法进行了比较,该方法具有代表性的临床细菌分离株的数据。比较了将不同细菌种类分离为不同簇的方法的效率。新的指数函数方法在将物种分离到不同簇中的能力以及为适当簇中的菌株赋予更大程度的同一性方面均优于其他方法。结果表明,细菌细胞脂肪酸的气液色谱法可有效地用于临床分离细菌的鉴定。但是,分析的有用性取决于所使用的比较方法及其应对数据变化的能力。

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