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Quantitative Methods Based on Twisted Nematic Liquid Crystals for Mapping Surfaces Patterned with Bio/Chemical Functionality Relevant to Bioanalytical Assays

机译：基于扭曲向列液晶的定量方法用于绘制与生物分析相关的具有生物/化学功能的表面

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We report methods for the acquisition and analysis of optical images formed by thin films of twisted nematic liquid crystals (LCs) placed into contact with surfaces patterned with bio/chemical functionality relevant to surface-based assays. The methods are simple to implement and are shown to provide easily interpreted maps of chemical transformations on surfaces that are widely exploited in the preparation of analytic devices. The methods involve acquisition of multiple images of the LC as a function of the orientation of a polarizer; data analysis condenses the information present in the stack of images into a spatial map of the twist angle of the LC on the analytic surface. The potential utility of the methods is illustrated by mapping (i) the displacement of a monolayer formed from one alkanethiol on a gold film by a second thiol in solution, (ii) coadsorption of mixtures of amine-terminated and ethyleneglycol-terminated alkanethiols on gold films, which leads to a type of mixed monolayer that is widely exploited for immobilization of proteins on analytic surfaces, and (iii) patterns of antibodies printed onto surfaces. These results show that maps of the twist angle of the LC constructed from families of optical images can be used to reveal surface features that are not apparent in a single image of the LC film. Furthermore, the twist angles of the LC can be used to quantify the energy of interaction of the LC with the surface with a spatial resolution of <10 µm. When combined, the results described in this paper suggest non-destructive methods to monitor and validate chemical transformations on surfaces of the type that are routinely employed in the preparation of surface-based analytic technologies.
机译：我们报告了获取和分析由扭曲向列液晶（LCs）薄膜形成的光学图像的方法，这些扭曲的向列液晶（LC）放置在与基于表面化验相关的具有生物/化学功能的表面上。该方法易于实施，并且显示出可提供易于解释的表面化学转化图，这些表面已广泛用于分析仪器的制备中。该方法包括根据偏振器的取向获取LC的多个图像。数据分析将图像堆栈中存在的信息浓缩为LC在分析面上的扭曲角的空间图。通过绘制（i）溶液中的第二个硫醇置换（i）在金膜上由一个链烷硫醇形成的单分子层的置换，（ii）胺端基和乙二醇终止的链烷硫醇的混合物在金上的共吸附，说明了该方法的潜在用途。膜，这导致了混合单层的一种类型，这种类型的单层被广泛用于将蛋白质固定在分析表面上，以及（iii）印刷在表面上的抗体图样。这些结果表明，由光学图像族构建的LC扭曲角图可用于揭示在LC膜的单个图像中不明显的表面特征。此外，LC的扭曲角可用于量化LC与表面相互作用的能量，空间分辨率小于10 µm。结合使用后，本文中描述的结果提出了一种非破坏性方法，用于监视和验证在制备基于表面的分析技术中通常使用的类型表面上的化学转化。

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