• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >A Continuous Assay of Acetate-Kinase Activity: Measurement of Inorganic Phosphate Release Generated by Hydroxylaminolysis of Acetyl Phosphate
【2h】

A Continuous Assay of Acetate-Kinase Activity: Measurement of Inorganic Phosphate Release Generated by Hydroxylaminolysis of Acetyl Phosphate

机译：醋酸激酶活性的连续测定：乙酰磷酸羟胺水解产生的无机磷酸盐释放量的测量

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acetate kinase, a member of the ASKHA (Acetate and Sugar Kinases, Hsp70, Actin) phosphotransferase superfamily is a central enzyme in prokaryotic carbon and energy metabolism. Recently extensive structural and biochemical studies of acetate kinase and related carboxylate kinases have been conducted. Analysis of the kinetic properties of wild-type and mutant enzymes has been impeded by the nature of the current assays for acetate kinase activity. These assays have the disadvantages of being either discontinuous or insensitive or of utilizing compounds that interfere with activity measurements. We have developed a novel continuous assay that depends on the purine nucleoside phosphorylase-based spectroscopic measurement of the inorganic phosphate generated by hydroxylaminolysis of one of the products of the acetate kinase reaction, acetyl phosphate. This assay has enabled a determination of the kinetic parameters of the Thermotoga maritima acetate kinase that indicates a lower Km for acetate than previously published.
机译：乙酸激酶是ASKHA（乙酸和糖激酶，Hsp70，肌动蛋白）磷酸转移酶超家族的成员，是原核碳和能量代谢中的核心酶。最近，已经进行了乙酸激酶和相关羧酸酯激酶的广泛结构和生化研究。当前的乙酸激酶活性测定法的性质已经阻碍了对野生型和突变型酶动力学特性的分析。这些测定法具有不连续或不灵敏或利用干扰活性测量的化合物的缺点。我们开发了一种新颖的连续测定法，该测定法基于嘌呤核苷磷酸化酶的光谱测定，该测定是由乙酸激酶反应产物之一乙酰磷酸的羟氨解产生的无机磷酸盐。该测定法能够确定马氏嗜热菌的乙酸激酶的动力学参数，该动力学参数表明乙酸钾的Km比以前公开的要低。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号