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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Restriction endonuclease analysis and ribotyping differentiate Pasteurella haemolytica serotype A1 isolates from cattle within a feedlot.
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Restriction endonuclease analysis and ribotyping differentiate Pasteurella haemolytica serotype A1 isolates from cattle within a feedlot.

机译：限制性核酸内切酶分析和核糖分型法可从饲养场的牛中区分溶血巴斯德氏菌血清型A1分离株。

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摘要

Pasteurella haemolytica serotype A1 isolates were collected from cattle within a feedlot during an outbreak of bovine respiratory disease. Genetic heterogeneity among the isolates was examined by restriction endonuclease analysis (REA), ribotyping, and analysis of plasmid content. The susceptibilities of isolates to several antibiotics were also examined. Five different REA patterns and three different ribotypes were observed among the isolates. Fifty percent of the isolates had an identical REA type, ribotype, and plasmid profile. Examination of the plasmid content of the isolates revealed that most (73%) carry a single plasmid which encodes beta-lactamase, 13.5% carry two plasmids, and 13.5% carry no plasmid. The data reveal the presence of genetic differences among isolates of P. haemolytica A1, associated with shipping fever pneumonia within a closed feedlot, and suggest that a combination of REA, ribotyping, plasmid analysis, and antibiotic susceptibility determination will be useful in analyzing the molecular epidemiology of this disease.
机译：在牛呼吸系统疾病暴发期间，从饲养场内的牛中收集了溶血巴斯德氏菌血清型A1分离株。通过限制性内切核酸酶分析（REA），核糖分型和质粒含量分析，检查了分离株之间的遗传异质性。还检查了分离株对几种抗生素的敏感性。在分离物中观察到五个不同的REA模式和三个不同的核糖型。 50％的分离株具有相同的REA类型，核糖型和质粒图谱。检查分离物的质粒含量发现，大多数（73％）携带单个编码β-内酰胺酶的质粒，13.5％携带两个质粒，而13.5％不携带质粒。数据揭示了溶血性疟原虫A1分离株之间存在遗传差异，与封闭饲养场内的运输性发热性肺炎有关，并表明REA，核糖分型，质粒分析和抗生素敏感性测定的组合将可用于分析分子这种疾病的流行病学。

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