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Identification of human herpesvirus 6 variants A and B by amplimer hybridization with variant-specific oligonucleotides and amplification with variant-specific primers.

机译：通过与变体特异性寡核苷酸的扩增子杂交并用变体特异性引物扩增鉴定人疱疹病毒6个变体A和B。

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Two distinct PCR-based procedures were evaluated for the detection and identification of human herpesvirus 6 (HHV-6) variants A and B in uncultured human samples. Variant-specific oligonucleotide hybridization (VSOH) is based on the amplification of two distinct regions of the HHV-6 genome, followed by hybridization of amplimers with variant-specific oligonucleotide probes. Variant-specific primer PCR (VSPP) is based on the amplification of each variant by using variant-specific primers. The study of 10 well-characterized HHV-6 strains allowed us to demonstrate the high sensitivity and specificity of both methods. With variant mixtures, however, some limitations of VSOH were evidenced and VSPP was required to obtain unambiguous results. The combination of VSOH and VSPP was applied to the direct study of 300 peripheral blood mononuclear cell samples from French subjects. HHV-6 was detected in 15 samples: 11 corresponded to variant B, 3 corresponded to variant A, and 1 corresponded to a mixture of both variants.

机译：评价了两种不同的基于PCR的程序，用于检测和鉴定未培养的人类样品中的人类疱疹病毒6（HHV-6）变体A和B。变体特异性寡核苷酸杂交（VSOH）基于HHV-6基因组两个不同区域的扩增，然后将扩增子与变体特异性寡核苷酸探针杂交。变异体特异性引物PCR（VSPP）基于使用变异体特异性引物对每种变异体的扩增。对10个特征明确的HHV-6菌株的研究使我们证明了这两种方法的高灵敏度和特异性。但是，对于不同的混合物，证明了VSOH有一些局限性，需要VSPP才能获得明确的结果。 VSOH和VSPP的结合用于直接研究法国受试者的300例外周血单个核细胞样品。在15个样品中检测到HHV-6：11个对应于变体B，3个对应于变体A，1个对应于两种变体的混合物。

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期刊名称 Journal of Clinical Microbiology
作者
J T Aubin; L Poirel; C Robert; J M Huraux; H Agut;
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作者单位

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年(卷),期 1994(32),10
年度 1994
页码 2434–2440
总页数 7
原文格式 PDF
正文语种
中图分类微生物学;
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专利

1. Identification of human herpesvirus 6 variants A and B by amplimer hybridization with variant-specific oligonucleotides and amplification with variant-specific primers. [J] . J T Aubin, L Poirel, C Robert, Journal of Clinical Microbiology . 1994,第10期

机译：通过与变体特异性寡核苷酸的扩增子杂交并用变体特异性引物扩增来鉴定人疱疹病毒6个变体A和B
2. Comparison of Variant-Specific Hybridization and Single-Strand Conformational Polymorphism Methods for Detection of Mixed Human Papillomavirus Type 16 Variant Infections [J] . Rebecca T. Emeny, John R. Herron, Long Fu Xi, Journal of Clinical Microbiology . 1999,第11期

机译：变异特异性杂交和单链构象多态性检测混合型人乳头瘤病毒16型变异感染的比较
3. Use of a novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant-specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia. [J] . Bienz M, Siles-Lucas M, Muller N Parasitology Research . 2001,第12期

机译：新颖的DNA熔解谱分析在鉴定来自贾第鞭毛虫无性系GS / M-83-H7株系的PCR扩增的基因组序列中的编码变体特异性表面蛋白的用途。
4. Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH) [C] . Michelle M. Packard, Maxim Shusteff, Evangelyn C. Alocilja Biosensing and nanomedicine IV . 2011

机译：基于介电电泳和无扩增荧光共振能量转移辅助原位杂交的新型快速，基于DNA的片上细菌鉴定系统（FRET-ISH）
5. HyPR-MS for Multiplexed and Splice Variant-Specific Discovery of RNA-Protein Interactomes [D] . Knoener, Rachel A. 2017

机译：HyPR-MS用于RNA-蛋白质相互作用组的多重和剪接变体特异性发现。
6. Comparison of Variant-Specific Hybridization and Single-Strand Conformational Polymorphism Methods for Detection of Mixed Human Papillomavirus Type 16 Variant Infections [O] . Rebecca T. Emeny, John R. Herron, Long Fu Xi, 1999

机译：变异特异性杂交和单链构象多态性检测混合型人乳头瘤病毒16型变异感染的比较
7. Comparison of Variant-Specific Hybridization and Single-Strand Conformational Polymorphism Methods for Detection of Mixed Human Papillomavirus Type 16 Variant Infections [O] . Rebecca T. Emeny, John R. Herron, Long Fu Xi, 1999

机译：用于检测混合人乳头瘤病毒16型变体感染的变体特异性杂交和单链构象多态性方法的比较
8. Use of Monoclonal Antibody to Immunochemically Characterize Variant-Specific Surface Coat Glycoprotein from Trypanosoma Rhodesiense [R] . Lyon, J. A., Pratt, J. M., Travis, R. W., 1980

机译：使用单克隆抗体免疫化学鉴定来自罗汉果的锥形变异特异性表面包膜糖蛋白

Identification of human herpesvirus 6 variants A and B by amplimer hybridization with variant-specific oligonucleotides and amplification with variant-specific primers.

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