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Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM™): Application to lipid-specific membrane protein topogenesis

机译：通过取代的半胱氨酸可及性方法（SCAM™）进行跨膜蛋白拓扑图谱分析：在脂质特异性膜蛋白拓扑学中的应用

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摘要

We provide an overview of lipid-dependent polytopic membrane protein topogenesis, with particular emphasis on Escherichia coli strains genetically altered in their lipid composition and strategies for experimentally determining the transmembrane organization of proteins. A variety of reagents and experimental strategies are described including the use of lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by substituted cysteine site-directed chemical labeling. Employing strains in which lipid composition can be controlled temporally during membrane protein synthesis and assembly provides a means to observe dynamic changes in protein topology as a function of membrane lipid composition.

机译：我们提供了脂质依赖性的多聚腺苷膜蛋白拓扑结构的概述，特别着重于在其脂质组成方面遗传改变的大肠杆菌菌株以及通过实验确定蛋白质的跨膜组织的策略。描述了多种试剂和实验策略，包括使用脂质突变体和巯基特异性化学试剂通过取代的半胱氨酸定点化学标记研究脂质依赖性和宿主特异性膜蛋白的拓扑结构。使用在膜蛋白合成和组装过程中可以暂时控制脂质组成的菌株，提供了一种观察蛋白质拓扑随膜脂质组成变化的动态变化的方法。

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期刊名称 other
作者
Mikhail Bogdanov; Wei Zhang; Jun Xie; William Dowhan;
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作者单位

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年(卷),期 -1(36),2
年度 -1
页码 148–171
总页数 46
原文格式 PDF
正文语种
中图分类
关键词
Membrane protein Cysteine scanning Maleimides Phospholipid Topogenesis Phosphatidylethanolamine Protein topology;

机译：膜蛋白;半胱氨酸扫描;马来酰亚胺;磷脂;拓扑结构;磷脂酰乙醇胺;蛋白质拓扑;

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专利

1. Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesis. [J] . Bogdanov M, Zhang W, Xie J, Methods: A Companion to Methods in Enzymology . 2005,第2期

机译：通过取代的半胱氨酸可及性方法（SCAM TM）的跨膜蛋白质拓扑图：应用于脂质特异性膜蛋白质拓扑发生。
2. Transmembrane topology of mammalian ORMDL proteins in the endoplasmic reticulum as revealed by the substituted cysteine accessibility method (SCAM (TM)) [J] . Davis Deanna, Suemitsu John, Wattenberg Binks Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics . 2019,第4期

机译：取代的半胱氨酸可粘附方法（ScAM（TM）透露，哺乳动物Ormd1蛋白哺乳动物Ormd1蛋白的跨膜拓扑
3. Topology of transmembrane proteins by scanning cysteine accessibility mutagenesis methodology. [J] . Zhu Q, Casey JR Methods: A Companion to Methods in Enzymology . 2007,第4期

机译：通过扫描半胱氨酸可及性诱变方法，跨膜蛋白的拓扑结构。
4. Helical net plots and surface lipophilicity mapping of transmembrane helices of integral membrane proteins:aids to 3D structure determination of integral membrane proteins [C] . Mire Zloh, Diego Esposito, William A.Gibbons the International Peptide Symposium . 2001

机译：整体膜蛋白透气纸螺旋螺旋网图和表面亲脂性测定：有助于3D结构测定整体膜蛋白的测定
5. Examining the extracellular topology of multidrug resistance protein 1 (MRP1): A substituted cysteine accessibility method. [D] . Clark, K. Fraser. 2005

机译：检查多药耐药蛋白1（MRP1）的细胞外拓扑：一种替代的半胱氨酸可及性方法。
6. Analysis of the membrane topology for transmembrane domains 7-12 of the human reduced folate carrier by scanning cysteine accessibility methods. [O] . Wei Cao, Larry H Matherly 2004

机译：通过扫描半胱氨酸可及性方法分析人还原叶酸载体跨膜域7-12的膜拓扑。
7. Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAMTM): Application to lipid-specific membrane protein topogenesis [O] . Mikhail Bogdanov, Wei Zhang, Jun Xie, 2005

机译：取代的半胱氨酸可接近方法（SCAMTM）的跨膜蛋白拓扑映射：在特异性特异性膜蛋白质拓扑中的应用

Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM™): Application to lipid-specific membrane protein topogenesis

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