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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Comparison of culture methods for monitoring Legionella species in hospital potable water systems and recommendations for standardization of such methods.
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Comparison of culture methods for monitoring Legionella species in hospital potable water systems and recommendations for standardization of such methods.

机译：监测医院饮用水系统中军团菌种的培养方法的比较并建议标准化这些方法。

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摘要

A lack of standardization of environmental monitoring techniques for Legionella spp. complicates the interpretation of results and comparisons of results from different institutions. A comparative assessment of techniques recommended by the Centers for Disease Control and Prevention, the Hygiene Institute (Graz, Austria), and our laboratory was performed. Variables investigated were sampling method (swabbing and collection of water samples [250 ml] before and after swabbing), method of concentration (none, filtration, and centrifugation), acid buffer treatment (no acid treatment, treatment for 3 min, and treatment for 15 min), and choice of medium (five formulations of buffered charcoal yeast extract agar with glycine, vancomycin, polymyxin B, anisomycin, or cycloheximide). Thirty-three sites in seven hospital buildings were studied. Recovery by swab correlated with recovery from water after swabbing (P < 0.05). However, the quantity of Legionella spp. recovered from swab specimens (mean, 3.0 x 10(4) CFU per swab) was greater than that recovered from water (mean, 4.7 x 10(3) CFU/250 ml). Filtration resulted in recovery rates (mean, 5.2 x 10(3) CFU/250 ml) higher than those by centrifugation (mean, 2.3 x 10(3) CFU/250 ml). Three minutes of acid buffer treatment to reduce overgrowth by commensal flora did not improve selectivity or sensitivity for Legionella spp. if glycine-containing selective media were used. Fifteen minutes of acid buffer treatment reduced recovery compared with that after a 3-min treatment. All glycine-containing media tested effectively inhibited background flora, but one selective medium containing dyes, glycine, vancomycin, and polymyxin B (DGVP) resulted in the greatest quantitative recovery of Legionella pneumophila.(ABSTRACT TRUNCATED AT 250 WORDS)
机译：军团菌属环境监测技术缺乏标准化。来自不同机构的结果解释和结果比较使解释变得复杂。对疾病控制和预防中心，卫生研究所（奥地利格拉茨）和我们实验室推荐的技术进行了比较评估。研究的变量包括采样方法（擦拭前后擦洗和收集水样[250 ml]），浓缩方法（无，过滤和离心），酸缓冲液处理（无酸处理，3分钟处理和15分钟），然后选择培养基（含甘氨酸，万古霉素，多粘菌素B，茴香霉素或环己酰亚胺的5种缓冲木炭酵母提取琼脂制剂）。研究了七个医院大楼中的33个站点。拭子恢复与拭子后从水​​中恢复相关（P <0.05）。但是，军团菌的数量。从拭子标本中回收的平均含量（每拭子3.0 x 10（4）CFU）大于从水中回收的标本（平均4.7 x 10（3）CFU / 250 ml）。过滤后的回收率（平均为5.2 x 10（3）CFU / 250 ml）比离心分离后的回收率（平均为2.3 x 10（3）CFU / 250 ml）高。三分钟的酸缓冲液处理可减少共生菌群的过度生长，但不能提高对军团菌的选择性或敏感性。如果使用了含甘氨酸的选择性培养基。与3分钟的处理后相比，酸缓冲液处理15分钟会降低回收率。所有测试的含甘氨酸的培养基均能有效抑制背景菌群，但一种包含染料，甘氨酸，万古霉素和多粘菌素B（DGVP）的选择性培养基可导致嗜肺军团菌的定量回收率最高（摘要以250字截短）。

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