首页> 美国卫生研究院文献>Animals : an Open Access Journal from MDPI >Effects of Gas Production Recording System and Pig Fecal Inoculum Volume on Kinetics and Variation of In Vitro Fermentation using Corn Distiller’s Dried Grains with Solubles and Soybean Hulls
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Effects of Gas Production Recording System and Pig Fecal Inoculum Volume on Kinetics and Variation of In Vitro Fermentation using Corn Distiller’s Dried Grains with Solubles and Soybean Hulls

机译:产气记录系统和猪粪便接种量对玉米蒸馏器加豆粕和豆壳干粮动力学及体外发酵变异的影响

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摘要

Various in vitro methodologies have been developed and used to estimate the digestibility of feed ingredients, such as corn distillers dried grains with solubles (cDDGS) and soybean hulls (SBH) which contain high concentrations of dietary fiber. This study evaluated two in vitro gas production recording systems (manual vs. automated) and two initial fecal inoculum volumes (30 vs. 75 mL) on the parameters of in vitro fermentation of cDDGS and SBH. The results showed that the use of 75-mL inoculum volume with 0.5 g substrate tended to reduce the variation of measurements compared to the 30-mL inoculum volume with 0.2 g substrate regardless of the gas production recording system. These findings suggest that using larger inoculum volume with more substrate increases the precision of measurements. Furthermore, the automated system decreases labor for conducting the assay.
机译:已经开发出各种体外方法,并用于估计饲料成分的消化率,例如玉米蒸馏器含可溶性干谷物(cDDGS)和大豆皮(SBH),其中含有高浓度的膳食纤维。这项研究根据cDDGS和SBH的体外发酵参数评估了两个体外产气记录系统(手动vs.自动)和两个初始粪便接种量(30 vs. 75 mL)。结果表明,与产气记录系统无关,与具有0.2 g底物的30 mL接种体积相比,使用具有0.5 g底物的75 mL接种体积倾向于减少测量值的变化。这些发现表明,使用更大的接种量和更多的底物可以提高测量精度。此外,自动化系统减少了进行测定的工作量。

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