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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Clinical Relevance of Direct Quantification of pp65 Antigenemia Using Flow Cytometry in Solid Organ and Stem Cell Transplant Recipients
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Clinical Relevance of Direct Quantification of pp65 Antigenemia Using Flow Cytometry in Solid Organ and Stem Cell Transplant Recipients

机译：用流式细胞术直接定量pp65抗原性在实体器官和干细胞移植受者中的临床意义

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A total of 1,305 blood samples from 85 solid organ transplant (SOT) recipients and 25 stem cell transplant (SCT) recipients at risk for cytomegalovirus (CMV) infection were prospectively collected and tested using the shell vial assay (SVA) and a leukocytic qualitative PCR (q-PCR). Of these, 462 specimens were further tested by direct quantification of CMV antigenemia by flow cytometry (FC-Ag), 125 were tested with a quantitative competitive PCR, and 200 were tested for pp65 antigenemia using the slide method (S-Ag). Laboratory data were statistically analyzed according to the presence of CMV-related symptoms. In SOT and SCT recipients, active CMV infection occurred in 63.5 and 36%, respectively, and CMV disease occurred in 53 and 24%, respectively. FC-Ag results correlated better with q-PCR and S-Ag than with SVA. The first test found to be positive during follow-up was FC-Ag in 73% of cases. In SOT recipients, FC-Ag showed the highest sensitivity and negative predictive value for the diagnosis of any grade of CMV disease. For FC-Ag, the threshold beyond which CMV disease was highly probable seemed to lie at 0.20% positive polymorphonuclear leukocytes. FC-Ag appears to be a useful test for the early detection of CMV infection and the prediction of CMV disease.
机译：前瞻性收集了来自85位有巨细胞病毒（CMV）感染风险的固体器官移植（SOT）受者和25位干细胞移植（SCT）受者的1,305份血样，并使用壳小瓶测定（SVA）和白细胞定性PCR进行了测试（q-PCR）。其中，通过流式细胞仪（FC-Ag）对CMV抗原血症进行直接定量，进一步检测了462个样本，通过定量竞争PCR检测了125个样本，并使用玻片方法（S-Ag）检测了200个样本中的pp65抗原血症。根据CMV相关症状的存在对实验室数据进行统计分析。在SOT和SCT接受者中，活动性CMV感染分别发生在63.5和36％，而CMV疾病分别发生在53和24％。与q-PCR和S-Ag相比，FC-Ag结果与SVA的相关性更好。随访期间发现阳性的第一个测试是FC-Ag，占73％。在SOT接受者中，FC-Ag对任何级别的CMV疾病的诊断显示出最高的敏感性和阴性预测值。对于FC-Ag，CMV疾病极有可能超过此阈值似乎位于0.20％阳性多形核白细胞上。 FC-Ag似乎是早期检测CMV感染和预测CMV疾病的有用测试。

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