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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Evaluation of the Wider System a New Computer-Assisted Image-Processing Device for Bacterial Identification and Susceptibility Testing
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Evaluation of the Wider System a New Computer-Assisted Image-Processing Device for Bacterial Identification and Susceptibility Testing

机译：评估更广泛的系统一种用于细菌鉴定和药敏试验的新型计算机辅助图像处理设备

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The Wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. It has been adapted to be able to read and interpret commercial MicroScan panels. Two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family Enterobacteriaceae, 25 nonfermentative gram-negative rods [NFGNRs], and 81 gram-positive cocci) were tested. In addition, 100 enterobacterial strains with known β-lactam resistance mechanisms (22 strains with chromosomal AmpC β-lactamase, 8 strains with chromosomal class A β-lactamase, 21 broad-spectrum and IRT β-lactamase-producing strains, 41 extended-spectrum β-lactamase-producing strains, and 8 permeability mutants) were tested. API galleries and National Committee for Clinical Laboratory Standards (NCCLS) microdilution methods were used as reference methods. The Wider system correctly identified 97.5% of the clinical isolates at the species level. Overall essential agreement (±1 log2 dilution for 3,719 organism-antimicrobial drug combinations) was 95.6% (isolates of the family Enterobacteriaceae, 96.6%; NFGNRs, 88.0%; gram-positive cocci, 95.6%). The lowest essential agreement was observed with Enterobacteriaceae versus imipenem (84.0%), NFGNR versus piperacillin (88.0%) and cefepime (88.0%), and gram-positive isolates versus penicillin (80.4%). The category error rate (NCCLS criteria) was 4.2% (2.0% very major errors, 0.6% major errors, and 1.5% minor errors). Essential agreement and interpretive error rates for eight β-lactam antibiotics against isolates of the family Enterobacteriaceae with known β-lactam resistance mechanisms were 94.8 and 5.4%, respectively. Interestingly, the very major error rate was only 0.8%. Minor errors (3.6%) were mainly observed with amoxicillin-clavulanate and cefepime against extended-spectrum β-lactamase-producing isolates. The Wider system is a new reliable tool which applies the image-processing technology to the reading of commercial trays for both bacterial identification and susceptibility testing.
机译：Wider系统是一种新近开发的计算机辅助图像处理设备，可用于细菌鉴定和抗菌药敏试验。它已被修改为能够读取和解释商用MicroScan面板。测试了244个新鲜的连续临床分离株（肠杆菌科的138个分离株，25个非发酵革兰氏阴性棒[NFGNR]和81个革兰氏阳性球菌）。此外，还有100个具有已知的β-内酰胺抗性机制的肠杆菌菌株（22个具有AmpC染色体的内酰胺酶菌株，8个具有A类β-内酰胺酶的菌株，21个产生广谱和IRTβ-内酰胺酶的菌株，41个广谱菌株测试了产生β-内酰胺酶的菌株和8个通透性突变体。 API画廊和临床试验国家标准委员会（NCCLS）的微稀释方法均用作参考方法。更广泛的系统在物种级别正确识别了97.5％的临床分离株。总体基本一致性（3,719种生物-抗微生物药物组合的±1 log2稀释度）为95.6％（肠杆菌科的分离物为96.6％； NFGNR的分离率为88.0％；革兰氏阳性球菌为95.6％）。肠杆菌科对亚胺培南（84.0％），NFGNR对哌拉西林（88.0％）和头孢吡肟（88.0％），革兰氏阳性分离物对青霉素（80.4％）的最低共识率最低。类别错误率（NCCLS标准）为4.2％（重大错误为2.0％，重大错误为0.6％，次要错误为1.5％）。八种针对具有已知β-内酰胺耐药机制的肠杆菌科细菌分离物的β-内酰胺抗生素的基本一致性和解释错误率分别为94.8％和5.4％。有趣的是，非常大的错误率仅为0.8％。轻微错误（3.6％）主要观察到阿莫西林-克拉维酸盐和头孢吡肟对产生广谱β-内酰胺酶的菌株的影响。 Wider系统是一种新的可靠工具，可将图像处理技术应用于商业托盘的读取，以进行细菌识别和药敏测试。

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