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Identification of Campylobacter jejuni C. coli C. lari C. upsaliensis Arcobacter butzleri and A. butzleri-Like Species Based on the glyA Gene

机译：基于glyA基因鉴定空肠弯曲菌大肠弯曲杆菌拉里弯曲杆菌upsaliensis弯曲杆菌Butzleri弓形杆菌和butzleri相似物种

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摘要

Currently, the detection and identification of Campylobacter and Arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. We have developed a PCR-hybridization strategy, wherein degenerate primers are used to amplify glyA fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, Arcobacter butzleri, and an A. butzleri-like species. Evaluation of this strategy with genomic DNA from different type strains suggests that this approach is both specific and sensitive and thus may be applicable in a diagnostic assay to identify and differentiate these highly related species.

机译：目前，弯曲杆菌属和弯曲杆菌属的检测和鉴定仍然很艰巨，这主要是由于跨物种的表型相似性和较窄的生化反应性谱。我们已经开发出一种PCR杂交策略，其中使用简并引物从样品中扩增glyA片段，然后对其进行物种特异性寡脱氧核糖核苷酸探针杂交，以鉴定和区分空肠弯曲杆菌，大肠杆菌，拉美氏梭菌，C upsaliensis，Arcobacter butzleri和A. butzleri样物种。用来自不同类型菌株的基因组DNA对该策略的评估表明，该方法既特异性又灵敏，因此可用于诊断测定以鉴定和区分这些高度相关的物种。

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期刊名称 Journal of Clinical Microbiology
作者
Shahnaz Tahihra Al Rashid; Irene Dakuna; Helena Louie; David Ng; Peter Vandamme; Wendy Johnson; Voon Loong Chan;
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作者单位

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年(卷),期 2000(38),4
年度 2000
页码 1488–1494
总页数 7
原文格式 PDF
正文语种
中图分类微生物学;
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专利

1. Identification of Campylobacter jejuni,C. coli, C. lari, C. upsaliensis,Arcobacter butzleri, and A. butzleri-Like Species Based on the glyA Gene [J] . Shahnaz Tahihra Al Rashid, Irene Dakuna, Helena Louie, Journal of Clinical Microbiology . 2000,第4期

机译：空肠弯曲杆菌的鉴定基于glyA基因的大肠埃希氏菌，乌拉氏梭菌，upsaliensis弯曲杆菌，布氏弓形杆菌和布氏芽孢杆菌样
2. Real-time TaqMan polymerase chain reaction-based genus-identification and pyrosequencing-based species identification of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus directly on stool samples [J] . PerssonS., PetersenH.M., JespersgaardC., Diagnostic microbiology and infectious disease . 2012,第1期

机译：直接在粪便样品上基于实时TaqMan聚合酶链反应的空肠弯曲杆菌，大肠弯曲杆菌，拉里弯曲杆菌，乌斯氏弯曲杆菌和胎儿弯曲杆菌的属鉴定和基于焦磷酸测序的菌种鉴定
3. Real-time TaqMan polymerase chain reaction-based genus-identification and pyrosequencing-based species identification of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus directly on stool samples [J] . PerssonS., PetersenH.M., JespersgaardC., Diagnostic microbiology and infectious disease . 2012,第1期

机译：实时Taqman聚合酶链反应的基于基于的基于纤维素的属鉴定和基于焦肌的物种鉴定，Camoncacter Jejuni，C.Coli，C.Lari，C. Upsaliensis和C.胎儿直接放在粪便样本上
4. Computational Characterization and Identification of Core Promoters of MicroRNA Genes in C. elegans , H. sapiens and A. thaliana [C] . Xuefeng Zhou, Jianhua Ruan, Guandong Wang, Joint Annual RECOMB Satellite Workshop on Systems Biology and Regulatory Genomics . 2007

机译：C.秀丽隐塞，H. Sapiens和A. Thaliana的微瘤基因核心启动子的计算表征及鉴定
5. Genetic Structure and Phenotypic Differences Among and Within Extant Populations of Chrysanthemum Arcticum L. and C. a. Subsp. Arcticum [D] . Liu, Yunjia. 2020

机译：菊花Arcticum L.和C.A的产卵群和群中的遗传结构和表型差异。副。 arcticum
6. Microarray-Based Identification of Thermophilic Campylobacter jejuni C. coli C. lari and C. upsaliensis [O] . Dmitriy Volokhov, Vladimir Chizhikov, Konstantin Chumakov, 2003

机译：基于微阵列空肠弯曲杆菌空肠弯曲杆菌的鉴定大肠杆菌C。lari和C. Upsaliensis
7. Microarray-Based Identification of Thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis [O] . Volokhov, Dmitriy, Chizhikov, Vladimir, Chumakov, Konstantin, 2003

机译：基于微阵列空肠弯曲杆菌空肠弯曲杆菌的鉴定大肠杆菌，C。lari和C. Upsaliensis

Identification of Campylobacter jejuni C. coli C. lari C. upsaliensis Arcobacter butzleri and A. butzleri-Like Species Based on the glyA Gene

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