首页> 美国卫生研究院文献>BMC Genomics >Transcriptome-wide association study identifies putative elicitors/suppressor of Puccinia graminis f. sp. tritici that modulate barley rpg4-mediated stem rust resistance
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Transcriptome-wide association study identifies putative elicitors/suppressor of Puccinia graminis f. sp. tritici that modulate barley rpg4-mediated stem rust resistance

机译:整个转录组关联研究确定了Puccinia graminis f。的推定诱导子/抑制子。 sp。调节大麦rpg4介导的茎锈病抗性的小麦

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摘要

Heatmap and dendrogram of 32 isolates with differential virulence on barley lines with and without the stem rust resistance genes RMRL and/or . Dendogram was obtained using hierarchical clustering (Fast Ward method) on the genotypic data generated for the 32 isolates used in this study. The colors of heatmap represents the relationship matrix [Identity by State (IBS)] value (shown on top right corner) that explain the degree of relatedness between the isolates. IBS with a value of 1 represents a perfect relationship and are dark red, while the decreasing IBS values represents increasing genetic diversity between two isolates compared. The isolates with an arrow on the left are the virulent RMRL (red arrows) virulent (green arrows) and avirulent RMRL and (black arrows) isolates selected for RNAseq analysis based on this diversity assay
机译:在有和没有茎锈病抗性基因RMRL和/或大麦的大麦品系上32种毒力不同菌株的热图和树状图。使用分层聚类(快速病房法)对本研究中使用的32个分离株产生的基因型数据获得树状图。热图的颜色表示关系矩阵[状态标识(IBS)]值(显示在右上角),该值解释了分离株之间的相关程度。值为1的IBS代表了一种完美的关系,呈深红色,而降低的IBS值则代表了所比较的两个分离株之间遗传多样性的增加。带有左侧箭头的分离株是强力RMRL(红色箭头),强力(绿色箭头)和无毒RMRL和(黑色箭头),基于这种多样性分析选择用于RNAseq分析的分离株

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