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Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

机译：从含有HNRPA2B1-CBX3泛在染色质开放元件（UCOE）核心片段的美容睡床转座子中持续表达转基因

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摘要

Progressive silencing of transposon vectors in CHO-K1 cells. Illustration of the experimental procedure used in this study including a schematic representation of the SB vector construct. Evaluation of stability of transgene expression in single cell CHO-K1 clones containing SB transposon vectors. On Day 0, puromycin was removed from the medium and eGFP expression monitored by flow cytometry at different time points for 7 weeks. Both the total percentage of eGFP-positive cells and the median fluorescence intensity is shown. Each line in the graphs represents the expression profile of a single clone over the course of the 7 weeks of continuous culture. LIR: Left inverted repeat, RIR: Right inverted repeat, CMV: Cytomegalovirus promoter, eGFP: enhanced Green fluorescent protein, IRES: Internal ribosomal entry site, pac: puromycin N-acetyl-transferase

机译：CHO-K1细胞中转座子载体的逐步沉默。本研究中使用的实验程序的图解，包括SB向量构建体的示意图。评估含有SB转座子载体的单细胞CHO-K1克隆中转基因表达的稳定性。在第0天，从培养基中除去嘌呤霉素，并在不同时间点通过流式细胞术监测eGFP的表达，持续7周。显示了eGFP阳性细胞的总百分比和中值荧光强度。图中的每条线代表在连续培养的7周过程中单个克隆的表达谱。 LIR：左反向重复，RIR：右反向重复，CMV：巨细胞病毒启动子，eGFP：增强的绿色荧光蛋白，IRES：内部核糖体进入位点，pac：嘌呤霉素N-乙酰基转移酶

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期刊名称 BMC Biotechnology
作者
Kristian Alsbjerg Skipper; Anne Kruse Hollensen; Michael N. Antoniou; Jacob Giehm Mikkelsen;
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年(卷),期 2019(19),-1
年度 2019
页码 -1
总页数 11
原文格式 PDF
正文语种
中图分类应用微生物学;
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专利

1. Ubiquitous Chromatin Opening Elements (UCOEs) effect on transgene position and expression stability in CHO cells following methotrexate (MTX) amplification [J] . Betts Zeynep, Dickson Alan J. Biotechnology Journal: Healthcare,Nutrition,Technology . 2016,第4期

机译：甲氨蝶呤（MTX）扩增后，泛在染色质开放元件（UCOEs）对CHO细胞中转基因位置和表达稳定性的影响
2. Optimization of short-term transgene expression by sodium butyrate and ubiquitous chromatin opening elements (UCOEs) [J] . de Poorter JJ, Lipinski KS, Nelissen RGHH, The journal of gene medicine . 2007,第8期

机译：通过丁酸钠和普遍存在的染色质开放元件（UCOE）优化短期转基因表达
3. Efficiency of different fragment lengths of the ubiquitous chromatin opening element HNRPA2B1-CBX3 in driving human CD18 gene expression within self-inactivating lentiviral vectors for gene therapy applications [J] . Gopinath Chitra, Chodisetty Sarvani, Ghosh Arkasubhra, Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2019,第期

机译：普遍存在染色质开口元素HNRPA2B1-CBX3在自灭活慢病毒载体中驱动人CD18基因表达的不同片段长度的效率
4. Sleeping Beauty: A DNA transposon system for therapeutic gene transfer in vertebrates. [D] . Yant, Stephen Russell. 2001

机译：睡美人：一种用于在脊椎动物中进行治疗性基因转移的DNA转座子系统。
5. Gene Therapy: Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells [O] . Fang Zhang, Susannah I. Thornhill, Steven J. Howe, -1

机译：基因治疗：慢病毒载体包含无增强子的染色质开放元件（UCOE）可在造血细胞中提供高度可再现和稳定的转基因表达
6. Correlation of DNA methylation with histone modifications across the HNRPA2B1-CBX3 ubiquitously-acting chromatin open element (UCOE) [O] . Allen, M L, Antoniou, M 2007

机译：HNRPA2B1-CBX3遍在起作用的染色质开放元件（UCOE）上的DNA甲基化与组蛋白修饰的相关性

Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

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