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The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow

机译：基于紫外和红外的激光显微解剖技术对激光显微解剖-反相蛋白质阵列工作流程中磷蛋白检测的影响

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摘要

Micrographs of HGSOC tissue sections before and after tumor epithelial cell harvest by laser microdissection. A representative H&E-stained section for each patient (n = 4) is shown at 0.3× (1st column) and 5× (2nd column) magnification. Tumor epithelium from tissue thin sections (10 µm) was enriched via ultraviolet laser microdissection (UV LMD) or infrared laser capture microdissection (IR LCM). Representative areas following microdissection of tumor epithelium are shown at 0.3× magnification (3rd column); the images shown are post-UV LMD

机译：通过激光显微切割收集肿瘤上皮细胞之前和之后的HGSOC组织切片的显微照片。以0.3倍（第一列）和5倍（第二列）的放大倍数显示了每个患者的代表性H＆E染色切片（n = 4）。通过紫外线激光显微切割（UV LMD）或红外线激光捕获显微切割（IR LCM）富集组织薄切片（10μm）的肿瘤上皮。肿瘤上皮显微切割后的代表性区域以0.3倍的放大倍数显示（第3列）；显示的图像是UV LMD后

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期刊名称 Clinical Proteomics
作者
Allison L. Hunt; Mariaelena Pierobon; Elisa Baldelli; Julie Oliver; Dave Mitchell; Glenn Gist; Nicholas W. Bateman; G. Larry Maxwell; Emanuel F. Petricoin; Thomas P. Conrads;
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作者单位

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年(卷),期 2020(17),-1
年度 2020
页码 -1
总页数 8
原文格式 PDF
正文语种
中图分类分子生物学;
关键词
Phosphoprotein; Laser microdissection; Laser capture microdissection; Reverse phase protein array; Proteomics;

机译：磷酸蛋白;激光显微切割;激光捕获显微切割;反相蛋白质阵列;蛋白质组学;

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专利

1. The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow [J] . Allison L. Hunt, Mariaelena Pierobon, Elisa Baldelli, Clinical proteomics. . 2020,第1期

机译：基于紫外线和红外线激光微生物切除技术对激光微粉术反相蛋白阵列工作流程中磷蛋白检测的影响
2. Impact of upfront cellular enrichment by laser capture microdissection on protein and phosphoprotein drug target signaling activation measurements in human lung cancer: Implications for personalized medicine [J] . Proteomics. Clinical applications . 2015,第9a10期

机译：激光捕获微粒对人肺癌中蛋白质和磷蛋白药物靶向信号激活测量的影响：对个性化医学的影响
3. Western blot detection of brain phosphoproteins after performing Laser Microdissection and Pressure Catapulting (LMPC). [J] . Maitre M, Roullot Lacarriere V, Piazza PV, Journal of Neuroscience Methods . 2011,第2期

机译：进行激光显微切割和压力弹射（LMPC）后，对脑磷蛋白的蛋白质印迹检测。
4. Broad-scale proteomic analysis from less than 3mg protein derived from human prostate epithelium isolated using laser capture microdissection [C] . Kristin West, Andra Frost, Zhi Huang, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：从使用激光捕获微量分离的人前列腺上皮衍生的少于3mg蛋白质的大规模蛋白质组学分析
5. Co-Phasing an Array of Segmented Lasers Via Interferometry and Heterodyne Phase Locking Methods [D] . Mitchell, Eric William. 2020

机译：通过干涉测量和外差相锁定方法共相配一系列分段激光器
6. Impact of Upfront Cellular Enrichment by Laser Capture Microdissection on Protein and Phosphoprotein Drug Target Signaling Activation Measurements in Human Lung Cancer: Implications for Personalized Medicine [O] . Baldelli Elisa, Haura Eric B., Crinò Lucio, -1

机译：激光捕获显微解剖前期细胞富集对人肺癌中蛋白质和磷酸蛋白药物靶信号激活测量的影响：对个性化医学的影响
7. Side-lobe Level Reduction of an Optical Phased Array Using Amplitude and Phase Modulation of Array Elements Based on Optically Injection-Locked Semiconductor Lasers [O] . Anh Hang Nguyen, Jun-Hyung Cho, Ho-Jun Bae, 2020

机译：使用基于光学注射锁定的半导体激光器的阵列元件的幅度和相位调制，光相控阵的侧凸电平降低光相控阵

The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow

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