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Metagenomic 16S rDNA amplicon data on bacterial diversity profiling and its predicted metabolic functions of varillales in Allpahuayo-Mishana National Reserve

机译:关于Allpahuayo-Mishana国家级自然保护区细菌多样性谱及其预测的代谢功能的超基因组16S rDNA扩增子数据

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摘要

The white-sands forests or varillales of the Peruvian Amazon are characterized by their distinct physical characteristics, patchy distribution, and endemism , . Much research has been conducted on the specialized plant and animal communities that inhabit these ecosystems, yet their soil microbiomes have yet to be studied. Here we provide metagenomic 16S rDNA amplicon data of soil microbiomes from three types of varillales in Allpahuayo-Mishana National Reserve near Iquitos, Peru. Composite soil samples were collected from very low varillal, high-dry varillal, and high-wet varillal. Purified metagenomic DNA was used to prepare and sequence 16S rDNA metagenomic libraries on the Illumina MiqSeq platform. Raw paired-endsequences were analyzed using the Metagenomics RAST server (MG-RAST) and Parallel-Meta3 software and revealed the existence of a high percentage of undiscovered sequences, potentially indicating specialized bacterial communities in these forests. Also, were predicted several metabolic functions in this dataset. The raw sequence data in fastq format is available in the public repository Discover Mendeley Data ( ). Also, is available at NCBI's Sequence Read Archive (SRA) with accession numbers SRX7891206 (very low varillal), SRX7891207 (high-dry varillal), and SRX7891208 (high-wet varillal).
机译:秘鲁亚马逊河的白沙森林或变种的特征是其独特的物理特征,斑驳的分布和特有性。已经对居住在这些生态系统中的专门动植物群落进行了大量研究,但尚未对其土壤微生物组进行研究。在这里,我们提供了秘鲁伊基托斯附近的Allpahuayo-Mishana国家级自然保护区的三种类型的varillales的土壤微生物组的宏基因组16S rDNA扩增子数据。复合土壤样品是从极低的,高干的和高湿的样品中收集的。纯化的宏基因组DNA用于在Illumina MiqSeq平台上制备16S rDNA宏基因组文库并对其进行测序。使用Metagenomics RAST服务器(MG-RAST)和Parallel-Meta3软件分析了原始的配对末端序列,发现存在大量未发现序列,这可能表明这些森林中存在专门的细菌群落。此外,还预测了该数据集中的几种代谢功能。公共存储库Discover Mendeley Data()中提供了fastq格式的原始序列数据。另外,在NCBI的序列读取档案(SRA)中也可用,其登录号为SRX7891206(非常低的品种),SRX7891207(高干燥的品种)和SRX7891208(高湿的品种)。

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