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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Sequence-Based Typing of Legionella pneumophila Serogroup 1 Offers the Potential for True Portability in Legionellosis Outbreak Investigation
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Sequence-Based Typing of Legionella pneumophila Serogroup 1 Offers the Potential for True Portability in Legionellosis Outbreak Investigation

机译：嗜肺军团菌血清群1的基于序列的分型提供了在军团菌病暴发调查中真正携带的潜力

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Seven gene loci of Legionella pneumophila serogroup 1 were analyzed as potential epidemiological typing markers to aid in the investigation of legionella outbreaks. The genes chosen included four likely to be selectively neutral (acn, groES, groEL, and recA) and three likely to be under selective pressure (flaA, mompS, and proA). Oligonucleotide primers were designed to amplify 279- to 763-bp fragments from each gene. Initial sequence analysis of the seven loci from 10 well-characterized isolates of L. pneumophila serogroup 1 gave excellent reproducibility (R) and epidemiological concordance (E) values (R = 1.00; E = 1.00). The three loci showing greatest discrimination and nucleotide variation, flaA, mompS, and proA, were chosen for further study. Indices of discrimination (D) were calculated using a panel of 79 unrelated isolates. Single loci gave D values ranging from 0.767 to 0.857, and a combination of all three loci resulted in a D value of 0.924. When all three loci were combined with monoclonal antibody subgrouping, the D value was 0.971. Sequence-based typing of L. pneumophila serogroup 1 using only three loci is epidemiologically concordant and highly discriminatory and has the potential to become the new “gold standard” for the epidemiological typing of L. pneumophila.
机译：分析了嗜肺军团菌血清群1的七个基因位点，作为可能的流行病学分型标记，以协助调查军团菌爆发。选择的基因包括四个可能具有选择性中性的基因（acn，groES，groEL和recA）和三个可能处于选择性压力下的基因（flaA，mompS和proA）。设计寡核苷酸引物，以扩增每个基因的279-763 bp片段。对10个特征明确的嗜肺乳杆菌血清群1分离株的7个基因座进行的初始序列分析显示出极好的可重复性（R）和流行病学一致性（E）值（R = 1.00; E = 1.00）。选择了显示最大区分度和核苷酸变异的三个基因座，flaA，mompS和proA，用于进一步研究。歧视指数（ D ）是使用79个不相关的菌株进行计算的。单个基因座的 D 值在0.767到0.857之间，所有三个基因座的组合产生的 D 值为0.924。当所有三个基因座与单克隆抗体亚组结合时， D 值为0.971。基于序列的 L键入。仅使用三个基因座的嗜肺菌血清群1在流行病学上是一致的，并且具有高度歧视性，并且有可能成为 L流行病学分型的新“黄金标准”。肺炎。

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