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Morphological characterization and phylogenetic relationships of Indochinese box turtles—The Cuora galbinifrons complex

机译:印支盒龟—中华(Cuora galbinifrons)复合体的形态特征和系统发育关系

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摘要

The members of the Indochinese box turtle complex, namely , , and , rank the most critically endangered turtle species on earth after more than three decades of over‐harvesting for food, traditional Chinese medicine, and pet markets. Despite advances in molecular biology, species boundaries and phylogenetic relationships, the status of the .  complex remains unresolved due to the small number of specimens observed and collected in the field. In this study, we present analyses of morphologic characters as well as mitochondrial and nuclear DNA data to reconstruct the species boundaries and systematic relationships within the .  complex. Based on principal component analysis (PCA) and statistical analysis, we found that phenotypic traits partially overlapped among , , and , and that and might be only subspecifically distinct. Moreover, we used the mitochondrial genome, , and nuclear gene under the maximum likelihood criteria and Bayesian inference criteria to elucidate whether could be divided into three separate species or subspecies. We found strong support for a sister relationship between and the other two species, and consequently, we recommend maintaining as a full species, and classifying and as subspecies of . These findings provide evidence for a better understanding of the evolutionary histories of these critically endangered turtles.
机译:经过三十多年对食品,中药和宠物市场的过度捕捞,印度支那箱形乌龟群的成员,和成为地球上最濒危的乌龟物种。尽管在分子生物学,物种边界和系统发生关系,分子的地位方面取得了进步。由于现场观察和收集到的标本数量很少,复杂的问题仍未解决。在这项研究中,我们目前进行形态特征分析以及线粒体和核DNA数据,以重建物种边界和系统内的系统关系。复杂。基于主成分分析(PCA)和统计分析,我们发现表型性状在,和之间部分重叠,并且可能只是亚种不同。此外,我们在最大似然标准和贝叶斯推断标准下使用线粒体基因组和核基因来阐明是否可以分为三个独立的物种或亚种。我们强烈支持与其他两个物种之间的姐妹关系,因此,我们建议将其保持为完整物种,并对其进行分类和分类。这些发现为更好地了解这些极度濒危的乌龟的进化史提供了证据。

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